16S rDNA phylogenetic analysis of 79 isolates that were not identified with the newly developed group-specific primers

Isolate(s)Microorganism with most similar sequence in database (accession no.)% Simi- larity
    A15, A46, A46, B02, B03, B08, B28, B49, C08, C25, C28, C35, D05, D06, D09, D10, D14, D15, D17, D18, D19, D21, D22, D26, D30, D33, D34, D41, D44, D49, E13, E19, E23Collinsella aerofaciens (AB011816)100
    C11, C15, C18, C41Collinsella aerofaciens (AB011816)99
    B17, D04, D23Collinsella aerofaciens (AB011816)98
Clostridium leptum subgroup
    A05, E10, E33Fusobacterium prausnitzii (X85022)99
    B26Fusobacterium prausnitzii (X85022)98
    A42, D50, E32, E34Fusobacterium prausnitzii (X85022)97
    C29, C44, D45Fusobacterium prausnitzii (X85022)96
    E47Fusobacterium prausnitzii (X85022)95
    C45Fusobacterium prausnitzii (X85022)94
    B20, B50, E07, E49Ruminococcus albus (L76598)95
    B01, B29Ruminococcus albus (L76598)94
    A02, F30Eubacterium desmolans (L34618)98
    E25Ruminococcus bromii (L76600)100
    B34Ruminococcus bromii (L76600)94
    A50Clostridium orbiscindens (Y18187)98
Disparate cluster
    B44, E09Bacteroides distasonis (M86695)100
    A45Bacteroides distasonis (M86695)97
    A14Bacteroides distasonis (M86695)94
    B18, B39Bacteroides distasonis (M86695)93
    C24Bacteroides putredenis (L16497)97
    E44Bacteroides putredenis (L16497)96
    A26Clostridium propionicum (X77841)96
    A38Clostridium aldrichii (X71846)92
    C13, D47Clostridium ramosum (M23731)93
    C46Megasphaera elsdenii (U95029)100
    F11Clostridium hiranonis (AB023971)100
    F22Anaerovibria glycerini (AJ010960)91