TABLE 1.

Overview of treatments of laboratory beach sand microcosms and molecular analyses of samples taken from the microcosms

Treatment codeNo. of microcosmsaOil addition (kg/m2)NaNO3 addition (% N [wt/wt] in oil)KH2PO4 addition (% P[wt/wt] in oil)Samples (days after fertilization) selected for:
DGGE analysisClone librariese
FOb,c3020.20, 2, 6, 13, 20, 260 (51), 6 (40), 26 (41)
0% Nc33.7000, 2, 6, 13, 20, 260 (50), 6 (45), 26 (78)
0.25% N13.70.250.0250, 2, 6, 13, 20, 26
0.5% N13.70.50.050, 2, 6, 13, 20, 26
0.75% Nd33.70.750.0756, 20, 26
1% N13.710.10, 2, 6, 13, 20, 26
2% N13.720.2
4% N13.740.40, 2, 6, 13, 20, 260 (66), 6 (67), 26 (60)
6% N13.760.6
10% N13.7101.00, 2, 6, 13, 20, 26
  • a Number of independent microcosms prepared.

  • b FO, fertilizer only.

  • c Molecular analyses of one microcosm from the triplicate.

  • d Molecular analyses of all three replicates.

  • e Sampling time in days; the value in parentheses is the number of clones screened.