TABLE 1.

Bacterial strains, bacteriophages, and plasmids used in this study

Strain, phage, or plasmidGenotype or relevant propertiesSource or reference
Bacterial strains
    E. coli DH5αMCRFmcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 supE44 λthi-1 gyrA96 relA1Invitrogen
    E. coli JM109 recA1 endA1 gyrA96 thi hsdR17 (rK mK+) supE44 relA1 Δ(lac-proAB) [F′ traD36 proAB lacIq ZΔM15]Laboratory stock
    E. coli JM109(DE3)JM109 with λDE3Promega
    E. coli LE392 hsdR514(rK mK+) supE44 supF58 lacY1 galK2 galT22 metB1 trpR55 (phage host; permissive for λgt11)Promega
    C. perfringens NCTC 3110Type B strain, propagation strain for φ3626, used as standard in the lysis assayNCTCa
Phages
    φ3626Isolated from C. perfringens ATCC 362643
    λΔSthfIn-frame deletion of S, single EcoRI site, cIts857, Ampr 36
    λΔSthf::Sλ S inserted into EcoRI site of λΔSthf 36
    λΔSthf::hol3626 hol3626 cloned into EcoRI site of λΔSthfThis study
Plasmids
    pACYC-IRL10Additional copies of ileX, argU, and leuW, Cmr 42
    pQE-303.4-kb coning and expression vector, T5 promoter/lac operator element; 5′ six-His tag coding sequence; AmprQiagen
    pSP722.4-kb cloning and expression vector; T7 promoter, AmprPromega
    pHPL3626 ply3626 cloned into BamHI-SalI sites of pQE-30This study
    pHPL3626ΔC5′ fragment (540 bp) of ply3626 cloned into pQE-30This study
    pSPply3626 ply3626 cloned into the EcoRI site of pSP72This study
  • a NCTC, National Collection of Type Cultures, London, United Kingdom.