TABLE 2.

Identity of the most abundant bacterial sequences and the proportional abundances of the phylogenetic groups in each librarya

Sampling datePutative identityTaxonomic group% of bacterial clones in the sample
May 17Flavobacterium/ChryseobacteriumCFB group40
FlexibacterCFB group24
Acidovoraxβ-Proteobacteria5
Bradyrhizobiumα-Proteobacteria4
Caulobacterα-Proteobacteria2
May 19Enterobacteriaceaeγ-Proteobacteria97
Acinetobacterγ-Proteobacteria1
May 22Pseudomonasγ-Proteobacteria20
Rhizobiumα-Proteobacteria20
Sphingomonasα-Proteobacteria13
Flavobacterium/ChryseobacteriumCFB group9
FlexibacterCFB group9
May 23Flavobacterium/ChryseobacteriumCFB group13
Methylobacteriumα-Proteobacteria10
FlexibacterCFB group9
Comamonadaceaeβ-Proteobacteria9
Burkholderiaβ-Proteobacteria8
Rhodobacterα-Proteobacteria5
May 25ArthrobacterActinobacteria14
HymenobacterCFB group13
PlanococcusFirmicutes7
Pseudomonasγ-Proteobacteria7
Comamonadaceaeβ-Proteobacteria6
Nitrosovibrioβ-Proteobacteria5
Sphingomonasα-Proteobacteria5
  • a Figure 2 details the total number of bacterial clones in each library. In all cases the sequences were >95% identical to the closest match in the database. In many cases, taxonomic identities could not be determined with a high degree of certainty, so sequences were only classified to the genus or family level. The phylogenetic placements of representative bacterial sequences assigned to the CFB group (Flavobacterium/Chryseobacterium and Flexibacter genera) and the Enterobacteriaceae are further detailed in Fig. 3.