Identity of the most abundant bacterial sequences and the proportional abundances of the phylogenetic groups in each librarya

Sampling datePutative identityTaxonomic group% of bacterial clones in the sample
May 17 Flavobacterium/Chryseobacterium CFB group40
Flexibacter CFB group24
Acidovorax β-Proteobacteria5
Bradyrhizobium α-Proteobacteria4
Caulobacter α-Proteobacteria2
May 19 Enterobacteriaceae γ-Proteobacteria97
Acinetobacter γ-Proteobacteria1
May 22 Pseudomonas γ-Proteobacteria20
Rhizobium α-Proteobacteria20
Sphingomonas α-Proteobacteria13
Flavobacterium/Chryseobacterium CFB group9
Flexibacter CFB group9
May 23 Flavobacterium/Chryseobacterium CFB group13
Methylobacterium α-Proteobacteria10
Flexibacter CFB group9
Comamonadaceae β-Proteobacteria9
Burkholderia β-Proteobacteria8
Rhodobacter α-Proteobacteria5
May 25 Arthrobacter Actinobacteria14
Hymenobacter CFB group13
Planococcus Firmicutes7
Pseudomonas γ-Proteobacteria7
Comamonadaceae β-Proteobacteria6
Nitrosovibrio β-Proteobacteria5
Sphingomonas α-Proteobacteria5
  • a Figure 2 details the total number of bacterial clones in each library. In all cases the sequences were >95% identical to the closest match in the database. In many cases, taxonomic identities could not be determined with a high degree of certainty, so sequences were only classified to the genus or family level. The phylogenetic placements of representative bacterial sequences assigned to the CFB group (Flavobacterium/Chryseobacterium and Flexibacter genera) and the Enterobacteriaceae are further detailed in Fig. 3.