TABLE 4.

Specificity of CowM2 and CowM3 qPCR assays

Sample typeLocaleaNo.CT for:
CowM2 multiplex assayCowM3 simplex assay (FAM)
CowM2 (FAM)IAC 50 copies (VIC)b
AvgSDAvgSDAvgSD
AlpacaWV234.00.9
Canadian GooseGA1234.40.24
CatWV1036.10.79
ChickenGA1034.30.26
Bovine herd 1WA1031.71.833.70.731.90.6
Bovine herd 2WA1030.80.3834.7129.460.86
Bovine herd 3WA833.20.6135.50.3231.80.71
Bovine herd 4WA1034.00.7835.80.4732.80.85
Bovine herd 5WY1034.20.3235.81.2732.30.5
Bovine herd 6WV1231.60.5834.60.5530.80.34
DeerWV634.50.14
DogWV1034.80.33
DuckGA1234.20.89
GoatDE1034.50.62
HorseWV534.40.52
HumanWV1634.40.17
PelicanFL534.41.24
PigWV1234.91.03
Sea gullGA1234.10.8
SheepDE1034.40.76
TurkeyOH734.60.41
WastewaterOH534.50.4
Total or avg20434.70.6
  • a WV, West Virginia; GA, Georgia; WY, Wyoming; DE, Delaware; FL, Florida; OH, Ohio.

  • b IAC detection levels indicate the absence of inhibition in all composites (expected CT for IAC spike, 35.4 ± 1.3 [Table 3]).