TABLE 1.

PCR primers and 18S rRNA-targeted oligonucleotide probes used in this study

Primer or probeaSequence (5′-3′)Target organism(s)% FormamideReference or source
18S-6-CIL-VAAYCTGGTTGATCCTGCCAGEukaryotesN/Ab15
18S-1511-CIL-RGATCCWTCTGCAGGTTCACCTACEukaryotesN/A15
BrB-F-171TCAAACCCGACTTTACGGAAGBrB ciliateN/AThis study
BrB-R-1721TGCAGGTTCACCTACGGAAACBrB ciliateN/AThis study
EUK1195GGGCATCACAGACCTGEukaryotes3016
BrB-754GTATTCGAGCCAAAGCCTBrB ciliate30This study
BrB-1461CGTATCCTTCCGGAACAGGTBrB ciliate30This study
NONEUBACTCCTACGGGAGGCAGCNoncomplementary304
  • a Probes labeled with the indocarbocyanine fluorochrome Cy5 (Thermo Hybaid, Germany).

  • b N/A, not applicable for PCR primers.