TABLE 2.

Deduced functions of the ORFs in the cylindrospermopsin gene cluster from C. raciborskii AWT205

NameEnzyme familySize (bp)Psi-BLAST similarity match% IdentityPutative function
cyrDPKS CrpB5,631ABM21570.1; Nostoc sp. strain ATCC 5378958PKS KS-AT-DH-KR-ACP
cyrFPKS CrpB4,074ABM21570.1; Nostoc sp. strain ATCC 5378968PKS KS-AT-ACP
cyrGCytosine deaminase/aminohydrolase/dihydro-orotase1,437BAF59909.1; Pelotomaculum thermopropionicum SI50Uracil ring formation
cyrIProlyl 4-hydroxylase831ABB06365.1; Burkholderia sp. strain 38343Hydroxylation of C-7
cyrKMatE Na+-driven multidrug efflux pump1,398EAW39051.1; Lyngbya sp. strain PCC 810665Exporter
cyrLTransposase750ABG50981.1; Trichodesmium erythraeum IMS10170Transposase
cyrHCytosine deaminase/aminohydrolase/dihydro-orotase1,431BAF59909.1; Pelotomaculum thermopropionicum SI50Uracil ring formation
cyrJBranched-chain amino acid aminotransferase780Trichodesmium erythraeum IMS10153Sulfotransferase
cyrAAmidinotransferase AoaA1,176AAX81898.1; Cylindrospermopsis raciborskii100Amidinotransferase
cyrBNRPS/PKS AoaB8,754AAM33468.1; Aphanizomenon ovalisporum97NRPS/PKS A domain, PCP, KS, AT, DH, Met, KR, ACP
cyrEPKS5,667ABA23591.1; Anabaena variabilis ATCC 2941362PKS KS-AT-DH-KR-ACP
cyrCPKS AoaC5,005AAM33470.1; Aphanizomenon ovalisporum97PKS KS-AT-KR-ACP
cyrMPartial transposase318ABG50981.1; Trichodesmium erythraeum IMS10170Transposase
cyrNAdenylylsulfate kinase (PAPS)600CAM76460.1; Magnetospirillum gryphiswaldense MSR-175Adenylylsulfate kinase (PAPS)
cyrOHypothetical protein1,548EAW46978.1; Nodularia spumigena CCY941474Regulator