TABLE 2.

Percent changes in protein expression in the variants relative to the wild type

ProteinFunctional annotation% Change in protein expression
OXCR1QACFGR2TOPR2
Antimicrobial resistance
    AcrAAcridine efflux pump135**151***−5
    AcrBRND family, acridine efflux pump113**158***−42
    TolCOuter membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes; role in organic solvent tolerance355***295***−64**
    ImpOrganic solvent tolerance protein9.30−60**
    OmpCOuter membrane protein 1b (ib;c); porin−66**−100†−100†
    OmpFOuter membrane protein 1a (ia;b;f); porin−100†−100†−100†
    OmpAPutative hydrogenase, membrane component−38*−44**−35*
    GrxCGlutaredoxin 3−100†−100†100
    GrxBGlutaredoxin 2317***217*−33
    TpxThiol peroxidase100100ND
    AhpFAlkyl hydroperoxide reductase, F52a subunit; detoxification of hydroperoxides−100†−100†100***
    AhpCAlkyl hydroperoxide reductase, C22 subunit; detoxification of hydroperoxides150**100−6
    SodBSuperoxide dismutase, iron−100†−100†ND
    TolBTol protein required for outer membrane integrity, uptake of group A colicins, and translocation of phage DNA to cytoplasm; may be part of multiprotein peptidoglycan recycling complex275*350**150*
    TsxNucleoside channel; receptor of phage T6 and colicin K138**8100***
    ElaBPutative inner membrane protein225**187*100*
    NfnBDihydropteridine reductase/oxygen-insensitive NAD(P)H nitroreductase300*340−100†
    FabIEnoyl-(acyl-carrier-protein) reductase (NADH)200*275ND
Virulence
    SipACell invasion protein−33133*67
    SipBCell invasion protein400*625**−100†
    SipCCell invasion protein157257**−14
    SipDCell invasion protein100100ND
    InvBAntigen surface presentation; secretory protein155462
    InvGInvasion protein; outer membrane100185**−75*
    InvHInvasion protein100ND100
    PrgHCell invasion protein−22−2211
    PrgKCell invasion protein; lipoprotein; may link inner and outer membranes−3313−46
    PrgICell invasion protein; cytoplasmicND100ND
    HilAInvasion gene transcription activatorND80−20
    SopBSalmonella outer protein; plasmid protein stabilization; homologous to IpgD of Shigella225*800*−100†
Metabolism: tricarboxylic acid cycle
    AcnBAconitate hydratase 2168***168***−47*
    SucB2-Oxoglutarate dehydrogenase (dihydrolipoyltranssuccinase E2 component)140**109**−18
    MdhMalate dehydrogenase243***202**−21
Metabolism: glycolysis
    AckAAcetate kinase A (propionate kinase 2)162250*ND
    PgiGlucose phosphate isomerase162*100*−12
    EnoEnolase100*80*−48*
    PykFPyruvate kinase I, fructose stimulated112**141*45*
Metabolism: phosphotransferase system
    PtsIGeneral phosphotransferase family (enzyme I) phosphoenolpyruvate-protein phosphotransferase377***322***11
    PtsHPhosphotransferase family; Hpr protein; phosphohistidinoprotein-hexose phosphotransferase166***133**−8
ATP synthase
    AtpCMembrane-bound ATP synthase, F1 sector, epsilon subunit−68*−64**−68*
    AtpDMembrane-bound ATP synthase, F1 sector, beta subunit−50*−57**−82**
    AtpFMembrane-bound ATP synthase, F0 sector, subunit b−54*−54*−70*
    AtpGMembrane-bound ATP synthase, F1 sector, gamma subunit−47*−43*−53*
    AtpHMembrane-bound ATP synthase, F1 sector, delta subunit−63**−45*−73**
DNA and RNA synthesis and repair
    RplY50S ribosomal subunit protein550***625**ND
    RpsF30S ribosomal subunit protein S6233**322**55
    RpsV30S ribosomal subunit protein S22166**133*33
    UdpUridine phosphorylase2,933***300**200*
    GuaAGMP synthetase380*140*80
    GcvPGlycine cleavage complex protein P; glycine decarboxylase550**5160
    PolADNA polymerase I; 3′-5′ polymerase; 5′-3′ and 3′-5′ exonuclease250*200ND
Motility
    CheASensory histitine protein kinase; transduces signal between chemosignal receptors and CheB and CheY−48*−25−56**
    CheYChemotaxis regulator; transmits chemoreceptor signals to flagellar motor components6683−50
    FlhAFlagellar biosynthesis; possible export of flagellar proteinsNDND−25
    FliCFlagellar biosynthesis; flagellin, filament structural protein48**100**−75***
LPS
    WzzBRegulator of length of O-antigen component of LPS chains100300**ND
    RfbGLPS side chain defect; CDP glucose 4,6-dehydratase240**160*0
Stress
    PhoPResponse regulator in two-component regulatory system with PhoQ; transcribes genes expressed at low Mg2+ concn (OmpR family)293***243**−18
    ClpXSpecificity component of ClpA-ClpP ATP-dependent serine protease; chaperone240*12060
    HtrAPeriplasmic serine protease Do; heat shock protein775**600**ND
    UspAUniversal stress protein A500**500***ND
  • a*, P < 0.05; **, P < 0.01; ***, P < 0.001; ND, not detected; †, protein that was found only in the proteome of the wild type; 100†, protein that was found only in the proteome of the disinfectant-cycled strain. Bold type indicates protein overexpression.