TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Source or reference
Strains
    E. coli
        DH5αF (φ80dlacZΔM15) Δ(lacZYA-argF)U169 hsdR17(rK mK+) recA1 endA1 relA1 (deoR phoA supE44 thi-1 gyrA9620
        XL1-BluehsdR17(rK mK+) recA1 endA1 gyrA96 thi-1 supE44 relA1 lac [F′ proAB laclqZΔM15 Tn10 (Tetr)]Stratagene
    S. carnosus
        TM300Wild type38
        TM300 (ΔsrtA)srtA::loxP-ermB-loxPThis study
        TM300 (ΔsrtA ΔermB)srtA::loxPThis study
    S. aureus
        RN4220NCTC8325-4 derivative, acceptor of foreign DNA22
        SA113 (ATCC 35556)NCTC8325 derivative, Δagr, 11-bp deletion in rbsU22
        SA113 (ΔarcA)arcA::lox66-aphAIII-lox71This study
        SA113 (ΔarcA ΔaphAIII)arcA::lox72This study
        SA113 (ΔarcABDCR)arcABDCR::lox66-aphAIII-lox71This study
        SA113 (ΔarcABDCR ΔaphAIII)arcABDCR::lox72This study
Plasmids
    pBT2cat bla; E. coli/staphylococcus shuttle vector, thermosensitive ori for staphylococci10
    pBT2-arcAAllelic replacement vector for S. aureus arcAThis study
    pBT2-arcOpAllelic replacement vector for S. aureus arcABDCRThis study
    pBT2-srtAAllelic replacement vector for S. carnosus srtAThis study
    pCrePAPpagA-cre; expression of cre in B. anthracis36
    pDG792Source of aphAIII19
    pEC2Source of ermB10
    pRAB1cat bla, PpagA-cre; pBT2 derivative; expression of cre in staphylococciThis study