TABLE 4.

Alleles for ospC and IGS sequences of B. burgdorferi obtained from each tick (one row per tick) collected from birds that were PCR positive for B. burgdorferia

ObservatoryPresence of indicated allele of:
ospC, determined by RLB and sequencingcIGS, determined by sequencingd
ABDFGHIKLO123567NV
Long Point, Ontariob++
++++
+++
++
++MXMXMXMXMXMXMX
+++
++++MXMXMXMXMXMXMX
++++
+++++
++
++
++
++
+NPNPNPNPNPNPNP
+NPNPNPNPNPNPNP
+NPNPNPNPNPNPNP
+NPNPNPNPNPNPNP
NPNPNPNPNPNPNPNPNPNP+
NPNPNPNPNPNPNPNPNPNP+
NPNPNPNPNPNPNPNPNPNP+
NPNPNPNPNPNPNPNPNPNP+
NPNPNPNPNPNPNPNPNPNPMXMXMXMXMXMXMX
Haldimand, OntariobNPNPNPNPNPNPNPNPNPNP+
Prince Edward Point,++
    Ontario++
++
++++MXMXMXMXMXMXMX
NPNPNPNPNPNPNPNPNPNP+
Atlantic, Nova Scotia++
  • a The IGS allele numbering scheme is that of Bunikis et al. (7). ospC alleles in bold are those that have been associated with disseminated infection in humans (51). NP, no PCR product was obtained; MX, polymorphic regions were seen in the sequences, suggesting mixed alleles; NV, a new IGS sequence variant that had almost equal identity to sequences of IGS alleles 2 and 4 (7).

  • b Long Point and Haldimand each comprise three bird observatories: Old Cut, Breakwater, and Tip; and Rockpoint, Selkirk, and Ruthven, respectively.

  • c For ospC, total numbers of alleles found were as follows: nine A alleles, five B alleles, two D alleles, one F allele, four G alleles, one H allele, two I alleles, seven K alleles, four L alleles, and three O alleles.

  • d For the IGS, total numbers of alleles found were as follows: four occurrences of allele 1, seven of allele 2, four of allele 3, one of allele 5, two of allele 6, two of allele 7, and one of the new variant.