TABLE 3.

Analysis of the frequency of genetic markers during the second round of must fermentations inoculated with tetrads from a sporulated culture of the yeasts from the seventh day of the Mix-1 (G418R = 45%, SMRR = 55%) and Mix-2 (G418R = 29%, SMRR = 71%) first-round fermentations of transgenic yeasts bearing the ime1Δ-linked G418R markera

Day of fermentationMix-1 tetrads (T15 = 3, T100 = 17)Mix-2 tetrads (T15 = 3, T100 = 17)
CFU/ml% of yeasts with indicated phenotypeCFU/ml% of yeasts with indicated phenotype
G418RSMRRG418RSMRRNone
48.7 × 10701006.5 × 10730700
62.4 × 10701001.5 × 10747467
113 × 10701002.1 × 107662410
142 × 10701003.9 × 10782108
191.5 × 10701003.8 × 107711811
  • a A total of 100 to 300 colonies were analyzed from each sample. The first sample was taken on the fourth day of fermentation because of the time needed for spore germination, mating, and yeast population growth. Phenotypes: G418R, resistant to G418; SMRR, resistant to smr; none, sensitive to both G418 and smr. The data are the mean values (percentages) of two independent experiments, and the standard errors were always <10% of the mean.