TABLE 1.

Bacterial strains and plasmids used in this study

Strain(s) or plasmidDescriptionaReference
E. coli strains
    K-12 BW25113lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD788
    K-12 BW25113 mutants (all)BW25113 Δ(gene) Ω Kmr2
    K-12 ATCC 25404Wild typeATCCb
    K-12 MG1655F lambda rfb-50 rph-1; lacks ilvG6
V. harveyi BB170BB120 luxN::Tn5 (negative for AI-1 sensor, positive for AI-2 sensor)58
Plasmids
    R1drd19Ampr Kmr Cmr Smr IncFII finO20
    pCM18Emr pTRKL2-PCP25RBSII-gfp3*-To-T125
  • a Ampr, Kmr, Cmr, Smr, and Emr denote ampicillin, kanamycin, chloramphenicol, streptomycin, and erythromycin resistance, respectively.

  • b ATCC, American Type Culture Collection.