TABLE 3.

Motility and aggregation assay results for E. coli BW25113 class I and class II mutants

MutantClassificationChange (n-fold) relative to wild-type measurementa in:
MotilityAggregation
Without R1drd19With R1drd19Without R1drd19With R1drd19
cpxAClass I0.4 ± 0.10.39 ± 0.091.04 ± 0.061.1 ± 0.1
rseAClass I2.8 ± 0.62.2 ± 0.51.13 ± 0.05267 ± 43
bssRClass I1.6 ± 0.41.7 ± 0.41.04 ± 0.041.14 ± 0.06
ompAClass I1.0 ± 0.11.1 ± 0.11.01 ± 0.051.3 ± 0.1
ymgAClass II7 ± 12.0 ± 0.41.01 ± 0.0347 ± 24
slpClass II1.2 ± 0.10.59 ± 0.071.00 ± 0.0337 ± 6
cstAClass II4.4 ± 0.43.2 ± 0.33.3 ± 0.59 ± 3
gatCClass II3.9 ± 0.83.6 ± 0.71.11 ± 0.028.5 ± 0.8
yqjDClass II2.2 ± 0.21.6 ± 0.20.94 ± 0.0310.2 ± 0.6
cpxRClass II4.4 ± 0.22.8 ± 0.11.38 ± 0.061.19 ± 0.07
cpxPClass II4.1 ± 0.73.6 ± 0.61.37 ± 0.024.6 ± 0.5
yagIClass II1.6 ± 0.31.4 ± 0.31.01 ± 0.0311 ± 1
ompCClass II2.1 ± 0.41.9 ± 0.51.10 ± 0.0513.2 ± 0.5
cspAClass II3.2 ± 0.62.9 ± 0.71.32 ± 0.079.3 ± 0.5
pspDClass II3.9 ± 0.73.4 ± 0.61.08 ± 0.0417 ± 3
pspBClass II1.0 ± 0.42.1 ± 0.41.2 ± 0.213 ± 3
ymgBClass II1.7 ± 0.62.0 ± 0.21.16 ± 0.029 ± 1
gadCClass II3.6 ± 0.82.1 ± 0.51.1 ± 0.112 ± 2
pspCClass II2.8 ± 0.52.1 ± 0.41.04 ± 0.044 ± 1
rseCClass II3.0 ± 0.52.4 ± 0.41.23 ± 0.0815 ± 2
ompFClass II4.3 ± 0.83.6 ± 0.71.5 ± 0.319 ± 2
  • a Motility was determined after 8 h at 37°C, and aggregation was determined after 7 h in LB medium at 37°C. Results are expressed as the ratio of the measurement for the indicated mutant to the measurement for the wild type. Data are the averages of results for two independent cultures, and one standard deviation is shown.