TABLE 2.

Transcript expression ratios of genes with significant differential expression in the heat shock, osmotic stress, and oxidative stress responsesa

GeneFold change (local Z score)Functional activity
Profile AProfile BProfile C
1 h3 h1 h3 h1 h3 h
Heat shock response genes
    clpA1.9 (1.5)2.9 (4.3)2.3 (2.9)1.5 (1.2)ATP-dependent molecular chaperone
    clpB9.4 (5.8)22.3 (8.8)5.1 (5.5)2.7 (3.1)4.3 (4.0)1.3 (1.7)Molecular chaperone
    clpP1.5 (1.7)1.91 (2.5)2.7 (2.2)1.5 (0.5)2.5 (2.3)0.7 (1.3)Serine protease
    dnaJ2.1 (1.5)2.5 (3.5)2.5 (2.7)2.1 (1.5)3.3 (2.9)1.5 (1.1)DnaJ/DnaK/GrpE chaperone system
    dnaK6.3 (3.7)6.4 (7.7)6.0 (3.5)2.3 (2.0)3.4 (2.5)0.8 (1.4)DnaJ/DnaK/GrpE chaperone system
    grpE5.3 (4.2)5.0 (6.0)4.9 (3.6)1.3 (0.9)2.0 (2.1)1.8 (1.4)DnaJ/DnaK/GrpE chaperone system
    hsIU3.1 (2.4)2.6 (5.3)1.9 (3.3)1.7 (1.1)3.4 (2.9)0.9 (0.1)Component of HsIVU protease
    hsIV2.1 (1.9)2.6 (3.9)2.5 (3.1)1.4 (1.9)3.3 (3.0)0.9 (1.6)Component of HsIVU protease
    htpG3.4 (2.2)3.4 (5.0)2.1 (3.6)2.0 (1.4)Molecular chaperone
    htpX1.8 (1.4)2.5 (3.5)1.8 (2.1)1.4 (1.3)Heat shock protein
    htrA2.1 (3.6)0.8 (1.7)Periplasmic serine protease
    ibpA5.7 (3.3)42.1 (7.8)18.1 (5.2)9.2 (4.2)3.7 (4.6)3.4 (3.3)Inclusion body protein
    ibpB5.1 (2.4)37.5 (9.4)14.2 (6.6)5.3 (3.3)6.8 (5.9)2.8 (4.0)Inclusion body protein
    ion2.1 (1.7)2.1 (3.2)ATP-dependent protease
    mopA3.4 (1.9)3.1 (4.9)4.5 (5.2)2.1 (1.2)2.5 (3.8)0.6 (1.6)Member of GroES molecular chaperone family
    mopB4.0 (2.2)4.9 (6.4)3.1 (4.2)2.7 (2.1)2.0 (3.6)1.1 (0.9)Member of GroES molecular chaperone family
Oxidative stress response genes
    acrA1.1 (0.6)3.5 (2.5)Periplasmic component of multidrug efflux pump
    ahpC1.4 (1.4)3.1 (3.4)1.1 (0.5)1.9 (2.2)Subunit of alkylhydroperoxide reductase
    dps1.4 (1.1)2.1 (2.3)2.9 (3.1)4.1 (4.0)2.2 (3.1)3.2 (12.1)Iron binding protein/DNA protection
    fumC1.1 (0.6)3.3 (2.4)Fumarase
    gor1.9 (0.3)2.8 (2.4)Unknown function
    grxA0.8 (0.2)3.1 (2.6)Glutaredoxin 1
    katE1.2 (2.6)3.0 (3.6)3.8 (3.2)Heme d synthase/hydroperoxidase
    katG1.1 (0.9)2.3 (2.7)2.6 (2.8)3.8 (3.1)Hydroperoxidase I
    poxB1.8 (2.6)2.2 (3.8)Acetoin synthesis/pyruvate oxidase
    sodA1.3 (0.3)1.8 (2.5)0.7 (1.0)1.9 (2.7)Superoxide dismutase (Mn)
    sodB1.3 (1.2)2.1 (2.4)0.8 (0.9)1.9 (2.0)Superoxide dismutase (Fe)
    soxS1.0 (0.4)1.8 (2.4)Transcriptional dual regulator
    sufA2.1 (3.1)3.6 (4.0)1.6 (2.1)2.4 (4.7)Fe-S cluster repair
    sufB1.4 (2.4)3.2 (3.3)1.6 (2.2)2.4 (6.5)Fe-S cluster repair
    sufC2.1 (2.3)2.4 (3.2)1.7 (2.2)2.2 (4.0)Fe-S cluster repair
    sufD1.8 (2.9)2.9 (3.7)1.9 (2.2)2.1 (3.7)Fe-S cluster repair
    trxC1.4 (0.9)1.9 (2.1)1.2 (1.3)2.4 (3.9)1.5 (0.3)4.2 (2.5)Thioredoxin 2
    zwf1.1 (0.1)2.6 (3.1)0.4 (−0.9)5.5 (3.4)Glucose 6-phosphate-1-dehydrogenase
Osmotic stress response genes
    betA1.5 (4.6)2.8 (4.1)1.3 (2.7)3.2 (3.7)Choline dehydrogenase
    betB1.7 (2.1)2.5 (3.5)1.6 (2.7)2.4 (2.8)Betaine aldehyde dehydrogenase
    betT0.8 (2.0)1.5 (2.7)Choline transporter
    kdpA1.8 (2.1)1.5 (−0.8)1.2 (1.8)1.9 (2.8)Subunit of potassium ion transporter
    osmB0.9 (2.8)3.2 (3.0)Osmotically inducible lipoprotein
    osmC1.7 (2.3)3.2 (2.7)3.3 (4.2)5.5 (6.0)3.2 (4.1)4.6 (3.5)Osmotically inducible peroxidase
    osmE2.4 (3.8)3.0 (4.5)1.3 (2.2)2.1 (3.3)Osmotically inducible protein
    osmY1.3 (1.7)3.2 (2.6)2.9 (3.8)6.6 (6.3)2.3 (4.6)3.6 (5.8)Osmotically inducible protein
    otsA1.8 (2.4)3.9 (5.2)1.2 (2.5)5.3 (4.5)Trehalose-6-phosphate synthase
    otsB1.9 (2.4)2.9 (4.6)1.3 (4.2)5.3 (4.9)Trehalose-6-phosphate phosphatase
    proP1.6 (1.6)2.1 (3.3)1.4 (2.3)1.9 (3.3)Proline/betaine MFS transporter
    proV−2.3 (−2.2)−1.7 (−2.6)Subunit of betaine transporter
  • a Profile A, 1- and 3-hour-postinduction transcript profile for the inactive-pathway control [E. coli DP10 containing pMevT(C159A) and pBAD18] relative to a preinduction profile; profile B, the mevalonate-producing strain (E. coli DP10 containing pBAD33MevT and pBAD18) 1- and 3-hour-postinduction transcript profile relative to a preinduction profile; and profile C, the mevalonate-producing strain's 1- and 3-hour-postinduction transcript profile relative to the inactive-pathway strain's 1- and 3-hour-postinduction transcript profile. The expression of genes not listed in a comparison did not change significantly in the respective comparison.