TABLE 1.

Strains used in this study

Strain or plasmidGenotype or descriptionSource or reference
Strains
    DH10BF mcrA Δ(mrr-hsdRMS-mcrBC) F80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu)7697 galU galK λ rpsL (Strr) nupGInvitrogen
    DP10DH10B Δ(araFGH) φ(ΔaraEp Pcp8-araE)51
Plasmids
    pBAD18Cloning vector containing Apr cassette, modified pBR322 origin with a truncated rop gene, araC, and PBAD22
    pBAD33Cloning vector containing Cmr cassette, pACYC184 origin, araC, and PBAD22
    pBAD33MevTpBAD33 containing atoB, ERG13, and tHMGR under the control of PBAD; Cmr44, 51
    pMevT(C159A)pBAD33MevT derivative containing ERG13(C159A)51