TABLE 1.

Phylogenetic affiliation and numbers of bacterial 16S rRNA clones retrieved from clone libraries generated from 13C-labeled RNA fractions

Phylogenetic groupaNo. of clonesT-RF length of clones (bp)b
MPJETTPMBF
Bacteroidetes1956207, 370, 371
Clostridium4614268, 70, 369, 371
Syntrophomonas1299
Syntrophothermus2372
Tepidanaerobacter71968
Desulfotomaculum11132, 244
Syntrophaceae31208
Clone MST group5248
Petrobacter3313
Synergistes2130
Spirochaetes4129
Coprothermobacter12132
Anaerobaculum62128
EM38204
OP71130
OP5372, 132
OP103127
Marine group A3207
WWE11305
NKB191210
Unidentified173
Total50505050
  • a Phylogenetic groups with cultivated representatives are named according to the taxonomic outline of Bergey's Manual of Systematic Bacteriology (4). Candidate phyla are named based on the review by Hugenholtz (9).

  • b Phylotypes that are represented by at least three clones and whose sequence length matches the lengths of specific T-RFs are highlighted in boldface, and others had T-RF lengths predicted using sequence data. Note that measured T-RFs are typically 0 to 3 bases shorter than the predicted T-RFs (24).