TABLE 2.

Putative genes in E. sakazakii O:1 or O:2 O-antigen gene cluster

ORFLocation in sequence (bp)G+C content (%)Size (aaa)Similar proteinSource (accession no.)% Identical/% similar (no. of aa)Putative function
O:1 cluster
    orf11-89153.4296GalFSalmonella enterica (AE017220)87/95 (295)UDP-glucose pyrophosphorylase
    orf21282-236150.3282RfbBShigella flexnerii (NP_707936)87/93 (273)dTDP-d-glucose 4,6 dehydratase
    orf32358-323040.9290RmlAEscherichia coli (AAT77170)81/90 (287)dTDP-glucose pyrophosphorylase
    orf43232-363335.8133WbtAEscherichia coli (ABK27315)55/73 (131)Putative isomerase
    orf53626-407831.8150FdtCEscherichia coli (AAT77171)49/58 (147)Acetyltransferase
    orf64079-501434.4311Xanthomonas axonopodis pv. Citri (NP_642021)45/62 (306)Hypothetical protein
    orf75011-611436.7367FdtBEscherichia coli (AAT77174)58/73 (365)Aminotransferase
    orf86111-737337.3420WzxEscherichia coli (AAT77175)46/67 (413)O-antigen flippase
    orf97373-831732.5314WbtEEscherichia coli (AAS73170)28/50 (254)Putative glycosyltransferase
    orf108356-963631.9426WzyEscherichia coli (AAZ85713)19/43 (272)O-antigen polymerase
    orf119623-1050136.7292WbgQShigella boydii (AAL27350)32/51 (281)Putative glycosyltransferase
    orf1210491-1125534.0254Shewanella baltica (ZP_01435692)55/68 (243)Glycosyltransferase
    orf1311469-1287554.3468GndEscherichia coli (ZP_00926655)93/97 (469)6-Phosphogluconate dehydrogenase
O:2 cluster
    orf11-59455.1197GalFEscherichia coli (AAS99158)83/90 (197)UDP-glucose pyrophosphorylase
    orf2986-206850.3360RmlBEscherichia coli (ABI98981)84/91 (360)dTDP-glucose 4,6-dehydratase
    orf32065-296749.5300RmlDShigella boydii (AAY23334)80/88 (298)dTDP-4-dehydrorhamnose reductase
    orf43017-389546.6292RmlASalmonella enterica (P55254)91/96 (292)dTDP-glucose synthase
    orf53899-444135.9180RfbCShigella boydii (YP_407363)81/90 (180)dTDP-4-dehydrorhamnose 3,5-epimerase
    orf64488-574430.2418RfbXEscherichia coli (NP_416541)27/52 (406)Putative O-antigen transporter
    orf75741-671833.2325Escherichia coli (ZP_01699967)22/42 (280)Glycosyltransferase
    orf86730-748529.4251WbsVShigella boydii (AAS98030)30/48 (241)Glycosyltransferase
    orf97475-847331.0332WzyShigella boydii (AAS98031)27/43 (317)O-antigen polymerase
    orf108493-933228.9279WbsWShigella boydii (AAS98032)30/47 (268)Glycosyltransferase
    orf119334-1034131.9335WbgMEscherichia coli (YP_541302)30/46 (110)Putative galactosyltransferase
    orf1210331-1109529.9254WbbLEscherichia coli (P36667)40/58 (257)Putative lipopolysaccharide biosynthesis glycosyltransferase
    orf1311462-1286854.4468GndEscherichia coli (ZP_00926655)93/97 (469)6-Phosphogluconate dehydrogenase
  • a aa, amino acids.