TABLE 2.

Putative genes in E. sakazakii O:1 or O:2 O-antigen gene cluster

ORFLocation in sequence (bp)G+C content (%)Size (aaa)Similar proteinSource (accession no.)% Identical/% similar (no. of aa)Putative function
O:1 cluster
    orf11-89153.4296GalF Salmonella enterica (AE017220 )87/95 (295)UDP-glucose pyrophosphorylase
    orf21282-236150.3282RfbB Shigella flexnerii (NP_707936 )87/93 (273)dTDP-d-glucose 4,6 dehydratase
    orf32358-323040.9290RmlA Escherichia coli (AAT77170 )81/90 (287)dTDP-glucose pyrophosphorylase
    orf43232-363335.8133WbtA Escherichia coli (ABK27315 )55/73 (131)Putative isomerase
    orf53626-407831.8150FdtC Escherichia coli (AAT77171 )49/58 (147)Acetyltransferase
    orf64079-501434.4311 Xanthomonas axonopodis pv. Citri (NP_642021 )45/62 (306)Hypothetical protein
    orf75011-611436.7367FdtB Escherichia coli (AAT77174 )58/73 (365)Aminotransferase
    orf86111-737337.3420Wzx Escherichia coli (AAT77175 )46/67 (413)O-antigen flippase
    orf97373-831732.5314WbtE Escherichia coli (AAS73170 )28/50 (254)Putative glycosyltransferase
    orf108356-963631.9426Wzy Escherichia coli (AAZ85713 )19/43 (272)O-antigen polymerase
    orf119623-1050136.7292WbgQ Shigella boydii (AAL27350 )32/51 (281)Putative glycosyltransferase
    orf1210491-1125534.0254 Shewanella baltica (ZP_01435692 )55/68 (243)Glycosyltransferase
    orf1311469-1287554.3468Gnd Escherichia coli (ZP_00926655 )93/97 (469)6-Phosphogluconate dehydrogenase
O:2 cluster
    orf11-59455.1197GalF Escherichia coli (AAS99158 )83/90 (197)UDP-glucose pyrophosphorylase
    orf2986-206850.3360RmlB Escherichia coli (ABI98981 )84/91 (360)dTDP-glucose 4,6-dehydratase
    orf32065-296749.5300RmlD Shigella boydii (AAY23334 )80/88 (298)dTDP-4-dehydrorhamnose reductase
    orf43017-389546.6292RmlA Salmonella enterica (P55254 )91/96 (292)dTDP-glucose synthase
    orf53899-444135.9180RfbC Shigella boydii (YP_407363 )81/90 (180)dTDP-4-dehydrorhamnose 3,5-epimerase
    orf64488-574430.2418RfbX Escherichia coli (NP_416541 )27/52 (406)Putative O-antigen transporter
    orf75741-671833.2325 Escherichia coli (ZP_01699967 )22/42 (280)Glycosyltransferase
    orf86730-748529.4251WbsV Shigella boydii (AAS98030 )30/48 (241)Glycosyltransferase
    orf97475-847331.0332Wzy Shigella boydii (AAS98031 )27/43 (317)O-antigen polymerase
    orf108493-933228.9279WbsW Shigella boydii (AAS98032 )30/47 (268)Glycosyltransferase
    orf119334-1034131.9335WbgM Escherichia coli (YP_541302 )30/46 (110)Putative galactosyltransferase
    orf1210331-1109529.9254WbbL Escherichia coli (P36667 )40/58 (257)Putative lipopolysaccharide biosynthesis glycosyltransferase
    orf1311462-1286854.4468Gnd Escherichia coli (ZP_00926655 )93/97 (469)6-Phosphogluconate dehydrogenase
  • a aa, amino acids.