TABLE 1

Strains and plasmids used and created during this study

Strain or plasmidCharacteristic(s)Source or reference
Strains
    Cyberlindnera fabianii CBS 5640Wild-type strainCBS
    Cyberlindnera jadinii CECT 1946Wild-type strainCECTa
    Kluyveromyces marxianus DSM 5422Wild-type strainDSMZ
    Wickerhamomyces anomalus DSM 6766Wild-type strainDSMZ
    Kluyveromyces lactis CBS 2359Wild-type strainCBS
    Kluyveromyces lactis CBS 2359 Δku80Disruption of KU8047
    Kluyveromyces lactis CBS 2359 Δku80 eat1-yegfpDisruption of KU80, Eat1 labeled with a C-terminal yEGFPThis study
    E. coli NEB5αCloning strainNew England Biolabs
Plasmids
    pUG75HygR marker template48
    pCY-3040-01yEGFP template49
    pYES2Invitrogen
    pYES2-KlaEat1-yEGFP-hphMX-1000Plasmid carrying the yegfp integration cassette targeting the 3′ end of the K. lactis eat1 locus with 1,000-bp homologous regionsThis study
  • a CECT, Colección Española de Cultivos Tipo (Spanish Type Culture Collection).