TABLE 3

Detailed information on the functional enzymes involved in MCCA formation

AbbreviationNameGeneDefinitionKO entryEC no.COGMTMGMT/MG
No. of hits%No. of hits%
Reverse β oxidation
    TLAThiolasefadAAcetyl-CoA acyltransferaseK006322.3.1.16COG0183780.01571,4290.04870.32
HADHBAcetyl-CoA acyltransferaseK075092.3.1.1610.000230.00011.96
    KCRKetoacyl-CoA reductasephbBAcetoacetyl-CoA reductaseK000231.1.1.36COG1028360.00722410.00820.88
    HCDHydroxyacyl-CoA dehydratasefadB3-Hydroxybutyryl-CoA dehydrogenaseK000741.1.1.157COG12501,4040.28202,0890.07133.96
fadJ3-Hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseK017821.1.1.35, 4.2.1.17, 5.1.2.3COG1250, COG102480.00162010.00690.23
fadN3-Hydroxyacyl-CoA dehydrogenaseK075161.1.1.35COG1250, COG1024290.00581,3380.04560.13
HADH3-Hydroxyacyl-CoA dehydrogenaseK000222.3.1.1640.0008290.00100.81
    ECREnoyl-CoA reductaseysiB, fadBEnoyl-CoA hydrataseK137674.2.1.17COG1024140.00281090.00370.76
fadJ3-Hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseK017821.1.1.35, 4.2.1.17, 5.1.2.3COG1250, COG102480.00162010.00690.23
paaF, echAEnoyl-CoA hydrataseK016924.2.1.17COG1024850.01712,8670.09780.17
echAEch hydrogenase subunit AK1408660.0012550.00190.64
crt3-Hydroxybutyryl-CoA dehydrataseK017154.2.1.177160.14385630.01927.49
    TESThioesteraseyciAAcyl-CoA thioesterase YciAK108063.1.2.−COG160770.0014710.00240.58
Fatty acid biosynthesis
    ACCAcetyl-CoA carboxylaseaccAAcetyl-CoA carboxylase carboxyl transferase subunit alphaK019626.4.1.2COG08251280.02571,2840.04380.59
accBAcetyl-CoA carboxylase biotin carboxyl carrier proteinK02160COG0511520.01043310.01130.93
accCAcetyl-CoA carboxylase, biotin carboxylase subunitK019616.4.1.2, 6.3.4.14COG04392560.05143,0590.10430.49
accDAcetyl-CoA carboxylase carboxyl transferase subunit betaK019636.4.1.2COG0777940.01891,0670.03640.52
    MATMalonyltransferasefasFatty acid synthase, bacteria typeK115332.3.1.−30.000630.00015.89
fabD[Acyl-carrier-protein] S-malonyltransferaseK006452.3.1.39COG03312830.05681,4360.04901.16
    KASKetoacyl-ACP synthasefasFatty acid synthase, bacteria typeK115332.3.1.−30.000630.00015.89
fabB3-Oxoacyl-[acyl carrier protein] synthase IK006472.3.1.41COG0304200.00402270.00770.52
fabF3-Oxoacyl-[acyl carrier protein] synthase IIK094582.3.1.179COG03041,2240.24583,9310.13411.83
fabH3-Oxoacyl-[acyl carrier protein] synthase IIIK006482.3.1.180COG03326170.12393,3410.11401.09
    KARKetoacyl-ACP reductasefasFatty acid synthase, bacteria typeK115332.3.1.−30.000630.00015.89
fabG3-Oxoacyl-[acyl carrier protein] reductaseK000591.1.1.100COG10281,0030.20145,2750.17991.12
    HADHydroxyacyl-ACP dehydratasefasFatty acid synthase, bacteria typeK115332.3.1.−30.000630.00015.89
fadZ3-Hydroxyacyl-[acyl carrier protein] dehydrataseK023724.2.1.59COG07642410.04841,0070.03431.41
    EAREnoyl-ACP reductasefasFatty acid synthase, bacteria typeK115332.3.1.−30.000630.00015.89
fabIEnoyl-[acyl carrier protein] reductase IK002081.3.1.9, 1.3.1.10COG06231500.03017770.02651.14
fabKEnoyl-[acyl carrier protein] reductase IIK023711.3.1.−COG20701630.03278840.03021.09
fabLEnoyl-[acyl carrier protein] reductase IIIK107801.3.1.−COG102860.00122160.00740.16