TABLE 2

Strains and plasmids used this study

Strain or plasmidRelevant characteristic(s)Source
S. cerevisiae
    BY4742MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0Laboratory stock
E. coliLaboratory stock
    DH5αsupE44 ΔlacU169(ϕ80dlacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1
    BL21(DE3)F ompT hsdSB(rB mB) gal(λ1857 ind1 sam7 nin5 lacUV5 T7 gene 1) dcmLaboratory stock
    BY4742 Δsul1Δsul1::BLEThis study
    BY4742 Δsul2Δsul2::LEU2This study
    BY4742 Δsul1 Δsul2Δsul1::BLE Δsul2::LEU2This study
    BY4742 Δmet5Δmet5::loxP Δrdl1::BLEThis study
    BY4742 Δmet5 Δrdl1Δmet5::BLEThis study
    BY4742 Δcys3Δcys3::BLEThis study
    Δcys3::CYS3Δcys3::YEplac195-CYS3This study
    Δmet15Δmet15::BLEThis study
    Δmet15::MET15Δmet15::YEplac195-MET15This study
    Δrdl1Δrdl1::BLEThis study
    Δrdl2Δrdl2::BLEThis study
    Δtum1Δtum1::HIS5This study
    Δych1Δmet15::LEU2This study
    Δuba4Δuba4::kanMXThis study
    Δrdl1 Δrdl2Δrdl1::loxP Δrdl2::BLEThis study
    Δrdl1 Δrdl2 Δtum1Δrdl1::loxP Δrdl2::BLE Δtum1::LEU2This study
    4kΔrdl1::loxP Δrdl2::BLE Δtum1::LEU2 Δych1::HIS3This study
    4k::RDL1Δrdl1::loxP Δrdl2::BLE Δtum1::LEU2 Δych1::HIS3 YEplac195-RDL1This study
    4k:: RDL2Δrdl1::loxP Δrdl2::BLE Δtum1::LEU2 Δych1::HIS3 YEplac195-RDL2This study
    4k::TUM1Δrdl1::loxP Δrdl2::BLE Δtum1::LEU2 Δych1::HIS3 YEplac195-TUM1This study
    4k::YCH1Δrdl1::loxP Δrdl2::BLE Δtum1::LEU2 Δych1::HIS3 YEplac195-YCH1This study
    4k::PSPEΔrdl1::loxP Δrdl2::BLE Δtum1::LEU2 Δych1::HIS3 YEplac195-PSPEThis study
    4k::GLPEΔrdl1::loxP Δrdl2::BLE Δtum1::LEU2 Δych1::HIS3 YEplac195-GLPEThis study
    4k::RDHAΔrdl1::loxP Δrdl2::BLE Δtum1::LEU2 Δych1::HIS3 YEplac195-RDHAThis study
    4k::DUF442Δrdl1::loxP Δrdl2::BLE Δtum1::LEU2 Δych1::HIS3 YEplac195-DUF442This study
    4k::SSEAΔrdl1::loxP Δrdl2::BLE Δtum1::LEU2 Δych1::HIS3 YEplac195-SSEAThis study
Plasmid
    YEplac195Multicopy plasmid for expression in yeastLaboratory stock
    pSH47Cre expression plasmidLaboratory stock
    pUG66BLE template plasmidaLaboratory stock
    pUG6kanMX template plasmidb
    pFA6a-GFP(S65T)-His3MX6HIS5 template plasmidcLaboratory stock
    pYE242ws-fapr-fapo17LEU2 template plasmiddLaboratory stock
    YEplac195-MET15MET15 in YEplac195, control by TEF1 promoterThis study
    YEplac195-CYS3CYS3 in YEplac195, control by own promoterThis study
    YEplac195-RDL1RDL1 in YEplac195, control by own promoterThis study
    YEplac195-RDL2RDL2 in YEplac195, control by own promoterThis study
    YEplac195-TUM1TUM1 in YEplac195, control by own promoterThis study
    YEplac195-YCH1YCH1 in YEplac195, control by own promoterThis study
    YEplac195-PSPEPSPE in YEplac195, control by TEF1 promoterThis study
    YEplac195-GLPEGLPE in YEplac195, control by TEF1 promoterThis study
    YEplac195-RDHARDHA in YEplac195, control by TEF1 promoterThis study
    YEplac195-DUF442DUF442 in YEplac195, control by TEF1 promoterThis study
    YEplac195-SSEASSEA in YEplac195, control by TEF1 promoterThis study
    pET30aExpression plasmid in E. coliLab stock
    pET30a-MET15MET15 in pET30a, control by IPTG-induced lac promoterThis study
    pET30a-RDL2RDL2 in pET30a, control by IPTG-induced lac promoterThis study
    pET30a-PDO2CpPDO2 in pET30a,control by IPTG-induced lac promoterLaboratory stock
    pET30a-RDL2 106ARDL2 in pET30a, 106 site was mutated to Ala, control by IPTG induced lac promoterThis study
    pET30a-RDL2 106SRDL2 in pET30a, 106 site was mutated to Ser, control by IPTG-induced lac promoterThis study
    pET30a-PSPEPSPE in pET30a, control by IPTG-induced lac promoterThis study
    pET30a-GLPEGLPE in pET30a, control by IPTG-induced lac promoterThis study
    pET30a-RDHARDHA in pET30a, control by IPTG-induced lac promoterThis study
    pET30a-DUF442CpDUF442 in pET30a, control by IPTG-induced lac promoterThis study
    pET30a-SSEASSEA in pET30a, control by IPTG-induced lac promoterThis study
  • a BLE, Zeocin resistance gene.

  • b kanMX, G418 resistance gene.

  • c HIS5 encodes a key enzyme for histone synthetase.

  • d LEU2, encoding a key enzyme for leucine synthetase.