TABLE 2

Summary of P. larvae and B. laterosporus identification by PCRa

StrainP. larvaeB. laterosporus
rpoBftsAthiGVagabond1Vagabond2rpoBftsAthiG
B. laterosporus
    40A1+^++
    Br1+^++
    Br2^+++
    Br3^^++
    Br4+^++
    Br5+^++
    Br6^+++
    Br7^^++
    Br9^+++
    Br12+NANA++
    Br13+NANA+++/−
    Br14^+++
B. brevis ATCC 8246++/−
Brevibacillus sp.NANA
P. larvae
    ATCC 9545++^^^
    PL309NANANA^^NANANA
    PL325NANANA^^NANANA
    PL335NANANA^^NANANA
    PL337NANANA^^NANANA
    PL338++^^^
    PL344NANANA^+NANANA
    PL345NANANA++NANANA
    PL357++^^+
P. alvei^
P. polymyxa^+
Paenibacillus sp.^^/−
  • a +, presence of the expected amplicon; ^, an occasional or consistent amplicon of an unexpected size generated in addition to the expected size for the primer pair due to off-target effects; −, lack of any amplicon as visualized by agarose gel electrophoresis; ∼, an amplicon would be consistently or occasionally produced, but not of the expected size; +/−, an amplicon that would appear sporadically but that would disappear as the annealing temperature was increased a few degrees above the given annealing temperature (to 60°C); ^/−, occasionally no product, and when there is a product, there is an occasional or consistent amplicon of an unexpected size generated in addition to the expected size; NA, that particular strain was not used in testing the specified primer set. Each result listed was obtained from two or more independent reactions.