TABLE 2

Oligonucleotide primersa used in this study

PrimerGeneNucleotide sequence
Quantitative RT-PCR
    CYP505D1-fCYP505D15′-CAAGTCCATTTGGGACGACT-3′
    CYP505D1-r5′-ACGAGACCAGGAGATGGAGA-3′
    CYP505D2-fCYP505D25′-ATGGAGAAGGCCAAGAACCT-3′
    CYP505D2-r5′-AGGAGGATGGACATGACGAC-3′
    CYP505D3-fCYP505D35′-ATGGAGAAGGCCAAGAACCT-3′
    CYP505D3-r5′-AGGAGGATGGACATGACGAC-3′
    CYP505D4-fCYP505D45′-ATGGAGAAGGCCAAGAACCT-3′
    CYP505D4-r5′-AGGAGGATGGACATGACGAC-3′
    CYP505D5-fCYP505D55′-ATGGAGAAGGCCAAGAACCT-3′
    CYP505D5-r5′-AGGAGGATGGACATGACGAC-3′
    CYP505D6-fCYP505D65′-CAGCTCCTCAAGTCCTACGG-3′
    CYP505D6-r5′-TCCTTCACAAGCTCCTCGAT-3′
    CYP505D7-fCYP505D75′-CATGGTCTGTGGTGTTGAGC-3′
    CYP505D7-r5′-CGAAGTTGTCGTCCTCCTTC-3′
    ACT1-fACT15′-CCAAGGCTAACCGTGAGAAG-3′
    ACT1-r5′-CACCAGAGTCGAGCACGATA-3′
Cloning for recombinant protein production
    CYP505D6-fCYP505D65′-ATGACGTCTACCATTCCGACGCCGCCGTCCAT-3′
    CYP505D6-r5′-CTATTCGAAGATATCGGTAGCGAA-3′
    CYP102A1-fCYP102A15′-ATGACAATTAAAGAAATGCCTCAGCCAAAAAC-3′
    CYP102A1-r5′-TTACCCAGCCCACACGTCTTTTGC-3′
Site-directed mutagenesis
    CYP505D6 V51Y-fCYP505D65′-TACGTCAGCTCGGCGAAGCTCAT-3′
    CYP505D6 V51Y-r5′-GAGCAGCTTCCTCCCGAGAA-3′
    CYP102A1 Y51V-fCYP102A15′-GTCTTATCAAGTCAGCGTCTAAT-3′
    CYP102A1 Y51V-r5′-GCGCGTTACACGACCAGGCG-3′