TABLE 1

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionaReference or source
Methylococcus capsulatus (Bath)
    Wild typeWild-type strain26
    SC-SacBSingle-crossover mutant constructed by integration of pJQYS1 in flanking region of xoxF of M. capsulatus (Bath)This study
    SC-PheS*Single-crossover mutant constructed by integration of pJQYS2 in flanking region of xoxF of M. capsulatus (Bath)This study
    ΔxoxFUnmarked mutant of xoxF (MCA0299)This study
    Bath (empty)Transformant of M. capsulatus (Bath) harboring pJN105 (vector control)This study
    Bath (pPheS*)Transformant of M. capsulatus (Bath) harboring pPheS*This study
Escherichia coli
    XL10-GoldHost for routine cloningAgilent
    WM6026Donor strain for conjugation46
Plasmids
    pJQ200skSuicide plasmid; Gmr, SacB45
    pUC57-pheS*pUC57 harboring pheS*This study
    pJQY3Suicide plasmid produced by substitution of sacB in pJQ200sk with pheS*This study
    pJQYS1DNA fragment containing upstream and downstream regions of xoxF ligated into BamHI site of pJQ200skThis study
    pJQYS2DNA fragment containing upstream and downstream regions of xoxF ligated into BamHI site of pJQY3This study
    pJN105Broad-host-range plasmid; Gmr, araC-PBAD promoter44
    pPheS*pJN105 carrying pheS* derived from M. capsulatus (Bath) under control of araC-PBAD promoterThis study
  • a Gmr, gentamicin resistance.