TABLE 5

Kinetic parameters of LipT6 variants in pNPL hydrolysis

LipT6 variantKm (10−2 mM)akcat (103 s−1)kcat/Km (103 s−1 · mM−1)
WTb7.9 ± 0.64.759
L184F8.4 ± 0.93.036
A187F5.4 ± 0.63.055
L360F5.1 ± 0.52.141
L184F/A187F5.1 ± 0.42.141
L184F/L360F1.1 ± 0.11.3116
A187F/L360F5.6 ± 0.71.630
  • a Values are means ± standard errors of the means.

  • b Obtained from Dror et al. (47).