TABLE 4

Unfolding temperature of LipT6 variants in methanol

LipT6 variantTm (°C) in:
Buffer60% methanol
WTa66.6 ± 0.138.9 ± 0.3
L184F67.5 ± 0.141.0 ± 0.2
A187F70.0 ± 0.243.0 ± 0.6
L360F69.9 ± 0.245.6 ± 0.2
L184F/A187F67.6 ± 0.146.1 ± 0.2
L184F/L360F65.5 ± 0.138.1 ± 0.1
A187F/L360F72.2 ± 0.146.2 ± 0.1
  • a Obtained from Gihaz et al. (50).