TABLE 1

Residues selected for mutagenesis on the basis of LipT6WT tunnel analysis

Tunnel no.aTunnel length (Å)bResidueGenerated mutation
121L184F, Y
A187F, Y
L360F, Y
2 and 3 (Y shape)13.5 and 13.9R215F, Y
411.5H154Y, W
57.8I11W
78.5F226Y
K330Y, W
88.8L380F
F268Y
  • a According to MOLE 2.0 automatic numbering; tunnels 6 and 9 were unchanged to maintain their rich hydrogen bond network.

  • b According to MOLE 2.0 job report.