TABLE 2

Primers and probes used for the study

NameaSequenceb
ddPCR
    Bl-04_F5′-CTT CCC AGA AGG CCG GGT-3′
    Bl-04_P5′-6-FAM/CGA AGA TGA/ZEN/TGT CGG AAC ACA AAC ACC CGG/3IABkFQ-3′
    Bl-04_R5′-CGA GGC CAC GGT GCT CAT ATA GA-3′
    HN019_F5′-TTC GAT GGT TCG CAC AGT GA-3′
    HN019_P5-6-FAM-AAA CAG GTC/ZEN/AAT CAG CGG CGC AGG GAG/3IABkFQ-3′
    HN019_R5′-GGT CTG ATG CCG CCT GAA AT-3′
SNPs
    Indel_2_FGCC GCA GAT CGA ATA CTG GG
    Indel_2_RAAG CGA CGA CCG AAT GCA AT
    Indel_4_FTCT GTT GCG GGA TGT CAT GC
    Indel_4_RGGC GAT TCA GGC GAA GTT CA
    Indel_7_FACA GAA GTA GGC GAG GGG AC
    Indel_7_RAAC ATC ACC GCC GAT GAA CC
    Indel_9_FGGA ACT TGG CAG ACG TCT CG
    Indel_9_RCTG TTG ACT CCG GCT GCA TT
    Indel_11_FGCA GAT CGC CCC ATT GAA CA
    Indel_11_RTCG GGT CTG CTC GAC ATT CT
    Indel_14A_FCAC ACG ATC GGA ACC AGT GG
    Indel_14A_RACG AAC GAA GTA GCC GAG GA
    Indel_14B_FCGC CGA ATC GCC ATA ATC CA
    Indel_14B_RCAG CCC GAA TCC ACT TGA CC
    Indel_17_FCTC AGT GTG CAC GCA CTC C
    Indel_17_RGAT GCA GGT TGA GCA AGG CG
    Indel_19_FCCT CGC TGT TTC GCT CTG AG
    Indel_19_RGGA AGG TGA CAT GCA GAC CG
    Indel_20_FAAC GAT CAT TTC CGC CAC CC
    Indel_20_RATG CTG TTC GAT GCG TTG GT
    Indel_22_FCAT CCA CAG CAG CCA ACT CA
    Indel_22_RCCT GAA CCA GAT TGC CAC CG
    Indel_23_FTTC GTC ACT GGA TCG CAA GC
    Indel_23_RTCC AAC AAA CTC ACC GTG GC
    Indel_27_FGCT CTT CGT CTT CGC GGT AC
    Indel_27_RCCG ACA ATC TGC GGC AAT GA
  • a ddPCR, droplet digital PCR; SNP, single-nucleotide polymorphism.

  • b ZEN, ZEN quencher (IDT); IABkFQ, Iowa Black fluorescence quencher.