TABLE 1

Overview of the fruiting body defense proteins (FBDPs) tested for toxicity against A. avenae and B. okinawaensisa

Lectin/defense protein (FBDP)bMolecular mass (kDa)OriginProtein familyLigand specificityToxicity against:GenBank accession no.References
A. aegyptiC. elegans
CGL216.7Coprinopsis cinereaGalectinGalβ(1,4)Glc, Galβ(1,4)GlcNAc, Galβ(1,4)FucToxicToxicAAF3473228, 48, 56
CCL215.3Coprinopsis cinereaRicin B-type lectinGlcNAcβ(1,4)[Fucα(1,3)]GlcNAcNontoxicToxicEU65985618, 57
TAP116.1Sordaria macrosporaActinoporin-like lectinGalβ(1,3)GalNAcToxicToxicCAH0368123, 28
MOA32.3Marasmius oreadesChimeric ricin B-type lectinGalα(1,3)GalToxicToxicAY06601335, 58
AAL33.4Aleuria aurantiaβ-Propeller lectinFucoseToxicToxicBAA1287128, 55, 59, 60
Tam115.1Pleurotus cornucopiaeBiotin-binding proteinBiotinNontoxicToxicAB10278433, 46
  • a Six different FBDPs from five fungal species were cloned and expressed in A. gossypii in order to test their toxicity toward fungal-feeding nematodes A. avenae and B. okinawaensis. The six selected FBDPs were previously shown to be toxic to at least one of the indicated bacterivorous or omnivorous model organisms.

  • b CGL2, Coprinopsis cinerea galectin 2; CCL2, Coprinopsis cinerea lectin 2; AAL, Aleuria aurantia lectin; MOA, Marasmius oreades agglutinin; TAP1, Sordaria macrospora transcript associated with perithecial development 1; Tam1, tamavidin 1.