TABLE 1

The strains and plasmids used in this research

Strain or plasmidDescriptionaReference or source
Strains
    A. tumefaciens GW4Wild type, As(III)-oxidizing strain24
    E. coli DH5αsupE44 lacU169(φ80lacZΔM15) hrdR17 recA1 endA1 gyrA96 thi-1 relA1Invitrogen
    E. coli S17-1F RP4-2-Tc::Mu aphA::Tn7 recA λpir lysogen; Smr TprInvitrogen
    E. coli BL21F ompT hsdSB (rB mB) gal dcm me131 (DE3) pLysS (Cmr)Invitrogen
    E. coli AW3110ΔarsRBC::cam F-IN(rrn-rrnE)12
Plasmids
    pUC19Ampr, clone and expression vectorMiaoLingBio
    pUC19-ArsKpUC19 containing ArsK coding regionThis study
    pLSP-KT2lacZKmr, oriV, lacZ-fusion vector used for lacZ fusion constructs8
    pLSP-arsR2-arsKpLSP-KT2lacZ containing arsR2-arsK promoterThis study
    pET-28(a+)Kmr, His6 tag expression vectorNovagen
    pET-ArsR2pET-28(a+) containing ArsR2 coding regionThis study
    pUC19-C97Cys97 site mutant of ArsKThis study
    pUC19-C183Cys183 site mutant of ArsKThis study
    pUC19-C318Cys318 site mutant of ArsKThis study
  • a Tc, tetracycline; Sm, streptomycin; Tp, trimethoprim; Cm, chloramphenicol; Km, kanamycin; Amp, ampicillin.