TABLE 2

The primers used in this research

PrimerDescriptionaUse
pUC19-ArsK-F5′-AAA AAGCTTGATGAGGGTGACGTCGTTG-3′Cloning for gene expression
pUC19-ArsK-R5′-AAA GGATCCGTCTCACTGTCAATGCCTC-3′
arsR2-arsK-F5′-GACATCCGACAGTCAGGG-3′Verification of cotranscription
arsR2-arsK-R5′-CACGGAGCCGATAGTCA-3′
arsK-hp-F5′-GGTGCCATCAGTGTCG-3′Verification of cotranscription
arsK-hp-R5′-CGCTGCAATCAGTCCC-3′
pLSP-arsK-F5′-AAA GAATTCGCCTTGACTTCGGTGAC-3′Cloning for lacZ reporter gene
pLSP-arsK-R5′-AAA GGATCCCCAACGCACGGACAAT-3′
pET28-arsK-F5′-AAA GGATCCATGGAAGAACGTCA-3′Overexpression of ArsK
pET28-arsK-R5′-AAA AAGCTTAGAGCCGATACGATGC-3′
arsK-EMSA-F5′-GTGTCTTGCCGTTTGC-3′Cloning for EMSA
arsK-EMSA-R5′-CCCTGACTGTCGGATGT-3′
C97-F5′-ACGCTCGCGATTGGCGCTTGGTCGC-3′Site mutant for ArsK
C97-R5′-GCCAATCGCGAGCGTCAGGGCCGCT-3′
C183-F5′-AACCTGTTTCTTGGTATGCCACTTC-3′Site mutant for ArsK
C183-R5′-ACCAAGAAACAGGTTCATTCCTGCA-3′
C318-F5′-GCCTTCGCCATCGGCCTTGGGCTCG-3′Site mutant for ArsK
C318-R5′-GCCGATGGCGAAGGCGACCGCACCC-3′
  • a The underlined sequences denote the restriction enzyme sites.