TABLE 3

Strains used in this study

Strain or plasmidDescriptionaReference or source
Strains
    Enterococcus faecium
        1,231,502Clade A1 isolate; also referred to as Efm50239
        1,230,933Clade A1 isolate39
        1,231,410Clade A1 isolate39
        1,231,408Hybrid clade A1-clade B isolate39
        1,231,501Clade A2 isolate39
        1,141,733Clade B isolate; also referred to as Efm73339
        Com12Clade B isolate39
        Com15Clade B isolate39
        Efm733ΔRMEfm733 with deletion of Efa733I (EFSG_05027-05029)This study
        Efm502ΔRMEfm502 with deletion of Efa502I (EFQG_01130-01132)This study
    Enterococcus faecalis
        OG1RFRifampicin- and fusidic acid-resistant derivative of E. faecalis OG146
        OG1SSpStreptomycin- and spectinomycin-resistant derivative of E. faecalis OG146
        OG1RF::efa502IOG1RF with Efa502I inserted at the GISEThis study
        OG1SSp::efa733IOG1SSp with Efa733I inserted at the GISEThis study
    Escherichia coli
        EC1000Plasmid propagation host42
        DH5αPlasmid propagation host
        STBL4Plasmid propagation hostFisher
        STBL4(pGEM-T)STBL4 with pGEM-T EasyThis study
        STBL4(pRB01)STBL4 with pGEM-T Easy vector containing EFSG_00659This study
Plasmids
    pGEM-T EasyCommercial plasmid for gene propagationPromega
    pLT06Temperature-sensitive plasmid41
    pAT28Shuttle vector for E. faecalis35
    pWH03Used for gene insertion in the enterococci14
    pRB01pGEM-T Easy vector with EFSG_00659This study
    pHA102pWH03 containing NotI-digested fragments of Efa733I M and S loci (EFSG_05028-05029)This study
    pHA103pWH03 containing NotI-digested fragments of Efa502I M and S loci (EFQG_01131-01132)This study
    pWH16pLT06 containing fragments upstream and downstream of Efa502IThis study
    pWH17pLT06 containing fragments upstream and downstream of Efa733IThis study
  • a EFSG_05027-05029 refers to EFSG_05027 through EFSG_05029 (etc.).