TABLE 1

Enzyme characteristics of purified AdhEa

ParameterValueb with substrate:
Acetyl-CoAEtOH
Sp act (U/mg protein)c7.92 ± 0.130.18 ± 0.03
Km (mM)0.12 ± 0.010.57 ± 0.07
kcat (s−1)23.33 ± 0.641.16 ± 0.04
kcat/Km (M−1 s−1)1.94E+052.04E+03
  • a Enzyme characteristics were measured at pH 7.0 and 30°C in 50 mM phosphate buffer.

  • b All values are means ± standard deviations from three repeats.

  • c One unit is defined as the enzyme activity catalyzing the transformation of 1 μmol NADH per min.