TABLE 3

Strains and plasmids used

Strain or plasmidCharacteristicsReference or source
Strains
    E. coli
        BL21(DE3)F ompT hsdSB(rB mB) gal dcm (DE3)Biomed
        DH5αF ϕ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK mK+) phoA supE44 λ thi-1 gyrA96 relA1Biomed
        BL21(DE3) (adhE)BL21(DE3) with expression vector pET28a-adhEThis work
        BL21(DE3) (lysR)BL21(DE3) with expression vector pET28a-lysRThis work
        BL21(DE3) (acrR)BL21(DE3) with expression vector pET28a-acrRThis work
        BL21(DE3) (rex)BL21(DE3) with expression vector pET28a-rexThis work
    Lactobacillus plantarumThis work
        WTStrain NF92, isolated from fermented grainsThis work
        △adhE mutantadhE disruption mutantThis work
        P-adhEadhE complemented strainThis work
        △acrR mutantacrR disruption mutantThis work
        P-acrRacrR complemented strainThis work
        AcrR+acrR high-level expression strain with pMG36c (M)-acrRThis work
        Rex+rex high-level expression strain with pMG36c (M)-rexThis work
        WT+WT strain with empty vector pMG36c (M)This work
Plasmids
    pET28aExpression vectorNovagen
    pET28a-adhEpET28a derivative with insertion of adhE coding regionThis work
    pET28a-lysRpET28a derivative with insertion of lysR coding regionThis work
    pET28a-acrRpET28a derivative with insertion of acrR coding regionThis work
    pET28a-rexpET28a derivative with insertion of rex coding regionThis work
    pEASY-BluntCloning vector, AmprTransGene
    Blunt-PadhEadhE promoter (PadhE) cloned in pEASY-Blunt vectorThis work
    pMG36c (M)Emr cassette of pMG36e was replaced by Cmr cassette, shuttle vectorThis work
    pMG36c (MK)P32 of pMG36c (M) was deleted for gene knockoutThis work
    pMG36c (M)-adhEComplemented vector, adhE under control of P32This work
    pMG36c (MK)-△adhEadhE knockout vector, pMG36c (MK) with Emr cassette flanked by adhE upstream and downstream regionsThis work
    pMG36c (MK)-△acrRacrR knockout vector, pMG36c (MK) with Ampr cassette flanked by acrR upstream and downstream regionsThis work
    pMG36c (MK)-acrRComplemented vector, acrR under control of its own promoterThis work
    pMG36c (M)-acrRacrR-overexpressing vectorThis work
    pMG36c (M)-rexrex-overexpressing vectorThis work