TABLE 1

Strains and plasmids used in this study

Strain or plasmidGenotype or descriptionaReference or source
Bacterial strains
    Escherichia coli
        DH5αF Φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK mK+) phoA supE44 λ thi-1 gyrA96 relA1Invitrogen
        K-12 JM109F′ traD36 proA+B+ lacIq Δ(lacZ)M15/Δ(lac proAB) glnV44 e14 gyrA96 recA1 relA1 endA1 thi hsdR17Promega
        2HB01JM109 carrying empty pB2HΔα and empty pB2HΔω (Ampr Cmr)This work
        2HB02JM109 carrying empty pB2HΔα and pB2HΔω_ferRLas (Ampr Cmr)This work
        2HB03JM109 carrying pB2HΔα_prbPLas and empty pB2HΔω (Ampr Cmr)This work
        2HB04JM109 carrying pB2HΔα_prbPLas and pB2HΔω_ferRLas (Ampr Cmr)This work
        2HB05JM109 carrying empty pB2HΔα and pB2HΔω_ferRLas Y3A (Ampr Cmr)This work
        2HB06JM109 carrying pB2HΔα_prbPLas and pB2HΔω_ferRLas Y3A (Ampr Cmr)This work
        2HB07JM109 carrying empty pB2HΔα and pB2HΔω_ferRLas V20A (Ampr Cmr)This work
        2HB08JM109 carrying pB2HΔα_prbPLas and pB2HΔω_ferRLas V20A (Ampr Cmr)This work
        2HB09JM109 carrying empty pB2HΔα and pB2HΔω_ferRLas C21A (Ampr Cmr)This work
        2HB10JM109 carrying pB2HΔα_prbPLas and pB2HΔω_ferRLas C21A (Ampr Cmr)This work
        2HB11JM109 carrying empty pB2HΔα and pB2HΔω_ferRLas V23A (Ampr Cmr)This work
        2HB12JM109 carrying pB2HΔα_prbPLas and pB2HΔω_ferRLas V23A (Ampr Cmr)This work
        2HB13JM109 carrying empty pB2HΔα and pB2HΔω_ferRLas D24A (Ampr Cmr)This work
        2HB14JM109 carrying pB2HΔα_prbPLas and pB2HΔω_ferRLas D24A (Ampr Cmr)This work
        2HB15JM109 carrying empty pB2HΔα and pB2HΔω_ferRLas C40A (Ampr Cmr)This work
        2HB16JM109 carrying pB2HΔα_prbPLas and pB2HΔω_ferRLas C40A (Ampr Cmr)This work
        2HB17JM109 carrying empty pB2HΔα and pB2HΔω_ferRLas D42A (Ampr Cmr)This work
        2HB18JM109 carrying pB2HΔα_prbPLas and pB2HΔω_ferRLas D42A (Ampr Cmr)This work
        2HB19JM109 carrying empty pB2HΔα and pB2HΔω_ferRLas C43A (Ampr Cmr)This work
        2HB20JM109 carrying pB2HΔα_prbPLas and pB2HΔω_ferRLas C43A (Ampr Cmr)This work
        2HB21JM109 carrying empty pB2HΔα and pB2HΔω_ferRLas V45A (Ampr Cmr)This work
        2HB22JM109 carrying pB2HΔα_prbPLas and pB2HΔω_ferRLas V45A (Ampr Cmr)This work
        BL21 (DE3)F ompT hsdS(rB mB) gal dcm (DE3) pRARENovagen
        ArcticExpress (DE3) RILE. coli B F ompT hsdS(rB mB) dcm+ gal λ (DE3) endA Hte [cpn10 cpn60] [argU ileY leuW] (Strr Tetr Gentr)Agilent
        EEPrbPBL21 (DE3) carrying p15TV-prbPLas (Ampr)21
        AEFerRArcticExpress (DE3) RIL carrying p15TV_ferRLas (Ampr)This work
        AEFerR V20AArcticExpress (DE3) RIL carrying p15TV_ferRLas V20A (Ampr)This work
        AEEFerR V23AArcticExpress (DE3) RIL carrying p15TV_ferRLas V23A (Ampr)This work
        AEEFerR C40AArcticExpress (DE3) RIL carrying p15TV_ferRLas C40A (Ampr)This work
        EERNAPDBL21 (DE3) carrying p15TV-rpoD, pACYCDuet-rpoB-rpoC, pRSFDuet-rpoZ-rpoA (Ampr Cmr Kanr)24
        ECPRPLKDH5α carrying pMiniT-PrplK (Ampr)24
    Liberibacter crescens BT-1Standard wild-type strain73
Plasmids
    pB2HΔαpACYCDuet-1Ωtac with E. coli β-galactosidase fragment lacking sequence for amino acids 11–41 (Δα) cloned in the BamHI-NcoI site (Cmr)38
    pB2HΔωpETDuet-1ΔSphIΩtac with E. coli β-galactosidase fragment lacking sequence for amino acids 789–1023 (Δω) cloned in the BamHI-NcoI site (Ampr)38
    pB2HΔα_prbPLaspB2HΔα with prbP gene from L asiaticus cloned in the BamHI-SphI site (Cmr)21
    pB2HΔω_ferRLaspB2HΔω with ferR gene from L asiaticus cloned in the BamHI-SphI site (Ampr)This work
    pB2HΔω_ferRLas Y3ApB2HΔω_ferRLas with Y3A (Ampr)This work
    pB2HΔω_ferRLas V20ApB2HΔω_ferRLas with V20A (Ampr)This work
    pB2HΔω_ferRLas C21ApB2HΔω_ferRLas with C21A (Ampr)This work
    pB2HΔω_ferRLas V23ApB2HΔω_ferRLas with V23A (Ampr)This work
    pB2HΔω_ferRLas D24ApB2HΔω_ferRLas with D24A (Ampr)This work
    pB2HΔω_ferRLas C40ApB2HΔω_ferRLas with C40A (Ampr)This work
    pB2HΔω_ferRLas D42ApB2HΔω_ferRLas with D42A (Ampr)This work
    pB2HΔω_ferRLas C43ApB2HΔω_ferRLas with C43A (Ampr)This work
    pB2HΔω_ferRLas V45ApB2HΔω_ferRLas with V45A (Ampr)This work
    p15TV-LExpression vector for purification of proteins by nickel affinity chromatography (Ampr)Cheryl Arrowsmith, (Addgene plasmid # 26093)
    p15TV_ferRLasferR gene from L asiaticus cloned in the BseRI site of p15TV-L (Ampr)This work
    p15TV-ferRLas V20Ap15TV_ferRLas with V20A (Ampr)This work
    p15TV-ferRLas V23Ap15TV_ferRLas with V23A (Ampr)This work
    p15TV-ferRLas C40Ap15TV_ferRLas with C40A (Ampr)This work
  • a Ampr, ampicillin resistance; Cmr, chloramphenicol resistance; Strr, streptomycin resistance; Tetr, tetracycline resistance; Gentr, gentamicin resistance; Kanr, kanamycin resistance.