TABLE 1

Microorganisms and plasmids used in this study

Strain or plasmidDescription or genotypeaSource or reference
Strains
    E. coli DH5αΦ80dlacZΔDM15 recA1 endA1 gyrA96 thi-1 hsdR17 (rK mK+) supE44 relA1 deoR Δ(lacZYA-argF)U169 phoAInvitrogen (Shanghai, China)
    E. coli BL21
    Bacillus velezensis SQR9Wild typeCGMCC no. 5808
    B. velezensis SQR9-gfpGFP-labeled SQR9 (Kanr)
    B. velezensis SQR9M6SQR9 Δsfp::cm60
SQR9 ΔggaAThis study
GFP-labeled SQR9 ΔggaA (Cmr)This study
SQR9 Δ3020This study
GFP-labeled SQR9 Δ3020 (Cmr)This study
SQR9 ΔgtaBThis study
GFP-labeled SQR9 ΔgtaB (Cmr)This study
Plasmids
    pTPCpMD19-T harboring the Pbc-pheS*-cat(PC) cassette54
    pNW33nCmr; MCSBGSC
    pNW33n-GFPThis study