TABLE 1

Enzymes expressed by “Candidatus Dichloromethanomonas elyunquensis” strain RM during growth with DCMa

Locus tag (AWM53)GeneBLAST resultsPredicted localizationbExpressed
EnzymeTaxonIdentity (%)
01188rdhAReductive dehalogenaseDehalococcoides mccartyi WBC-252PeriplasmYes
01801rdhAReductive dehalogenaseDehalobacter sp. UNSWDHB72MembraneYes
Dehalobacter sp. DCA71
00864rdhAReductive dehalogenaseDehalobacter sp. FTH172CytoplasmND
01380mtrHTetrahydromethanopterin S-methyltransferase subunit HDehalobacterium formicoaceticum91CytoplasmYes
01379mtaAMethylcobamide:CoM methyltransferase MtaADehalobacterium formicoaceticum94CytoplasmYes
02085mtsAMethylated-thiol coenzyme M methyltransferaseDehalobacterium formicoaceticum92CytoplasmYes
02086mtbCCorrinoid methyltransferaseDehalobacterium formicoaceticum86CytoplasmYes
01363fhsFormyl-THF ligaseSyntrophobotulus glycolicus85MembraneYes
00605fchAMethenyl-THF cyclohydrolaseDesulfosporosinus youngiae69MembraneYes
00606folDBifunctional: 5,10-methylene-THF dehydrogenase, 5,10-methylene-THF cyclohydrolaseDesulfosporosinus acidiphilus72CytoplasmYes
00219metFMethylene-THF reductase [NAD(P)H]Dehalobacter sp. FTH166CytoplasmYes
00225acsECarbon monoxide dehydrogenaseDehalobacter sp. FTH193CytoplasmYes
00220cooSCarbon monoxide dehydrogenase catalytic subunitDehalobacter restrictus DSM 945580MembraneYes
00226acsBAcetyl-CoA decarbonylase/synthaseDehalobacter sp. FTH185MembraneYes
  • a The most closely related proteins and predicted localization (i.e., periplasm, membrane, and cytoplasm) of strain RM enzymes are also shown. ND, not detected.

  • b Based on PSORT and SignalP 4.1.