TABLE 2

Primers and TaqMan probes used to quantify 16S rRNA genes

Primer/probeSequence (5′–3′)Target(s)Reference(s) or source
Set 1
    Bac1055YFATGGYTGTCGTCAGCTBacteria58, 78
    Bac1392RACGGGCGGTGTGTACBacteria79
    Bac1115-probeFAM-CAACGAGCGCAACCC-MGBBacteria79, 80
Set 2
    DhbRM114FCCGCAACGAGCGCAACStrain RMThis study
    DhbRM1168RTTGTCACCGGCAGTCTATCCAStrain RMThis study
    Dhc-H-PR2-probeFAM-CAGTTACCAGCACGTAAA-MGBStrain RMThis study
Set 3
    Mtgen835FGGGRAGTACGKYCGCAAGMethanogensThis study
    Mtgen918RGAVTCCAATTRARCCGCAMethanogensThis study
    Mtgen831-probeFAM-CCAATTCCTTTAAGTTTCA-MGBMethanogensThis study