TABLE 1

Strains and plasmids used in this study

Strain or plasmidRelevant genotypeaNote (reference)Source
Strains
    SD100WT KazusaWild type, amotile (81)Gift from Willem Vermaas lab
    SD500WT ParisWild type, motile (81)Pasteur Cyanobacterial Collection
    SD515Paris sll1581::Kmr-sacBwza mutantThis study
    SD517Kazusa sll1581::Kmr-sacBwza mutantThis study
    SD519Kazusa slr0162-slr0163::Kmr-sacBpilC mutantThis study
    SD520Paris slr0162-slr0163::Kmr-sacBpilC mutantThis study
    SD522Paris sll1951::Kmr-sacBS-layer mutantThis study
    SD523Kazusa sll1951r::Kmr-sacBS-layer mutantThis study
    SD546Kazusa sll0923::Kmr-sacBwzc mutantThis study
    SD577Kazusa slr0162-slr0163::Kmr-sacB pΨ552pilC mutant with extrachromosomal pilCThis study
Plasmids
    pGEM 3ZpUC18 derivativeCloning vectorPromega Corporation
    pΨ540sll1581 up::Kmr-sacB::sll1581 downSuicide vector for constructing SD515 and SD517This study
    pΨ541slr0162-slr0163 up::Kmr-sacB::slr0162-slr0163 downSuicide vector for constructing SD519 and SD520This study
    pΨ543sll1951 up::Kmr-sacB::sll1951 downSuicide vector for constructing SD522 and SD523This study
    pΨ546sll0923 up::Kmr-sacB::sll0923 downSuicide vector for constructing SD546This study
    pΨ552slr0162-slr0163Expression vector for pilCThis study
    pΨ568RSF1010 derivativeExpression vectorGift from Soo-Young Wanda
    pPSBA2KSpSL1180 derivative (Pharmacia Biotech)Source of Kmr-sacB markers (106)Gift from Willem Vermaas lab
  • a “Up” and “down” refer to regions flanking the gene of interest targeted for replacement (upstream and downstream, respectively; see Materials and Methods).