TABLE 3

Amplification primers used in this study

Target genesPrimerSequence (5′–3′)Reference
Bacterial 16S rRNAEub338CCTACGGGAGGCAGCAG61
Eub518ATTACCGCGGCTGCTGG62
515FGTGCCAGCMGCCGCGG63
907RCCGTCAATTCMTTTRAGTTT64
Fungal ITSITS1fTCCGTAGGTGAACCTGCGG65
5.8sCGCTGCGTTCTTCATCG66
ITS3GCATCGATGAAGAACGCAGG67
ITS4RTCCTCCGCTTATTGATATGC65
R. solani ITSST-RS1AGTGTTATGCTTGGTTCCACT68
ITS4RTCCTCCGCTTATTGATATGC65