TABLE 1

Strains and plasmids used in this study

Strain or plasmidDescription and/or genotypeaReference or source
Bacteria
    E. piscicida
        PPD130/91Wild type, Kms Colr Amps, LD50 = 105.02
        ΔeseB strainPPD130/91 with in-frame deletion of eseB15
        ΔeseC strainPPD130/91 with in-frame deletion of eseC23
        ΔeseD strainPPD130/91 with in-frame deletion of eseDThis study
        ΔeseC/pJN-eseC strainΔeseC mutant with pJN105-eseCThis study
        ΔeseC/pACYC-eseC-2HA strainΔeseC mutant with pACYC184-eseC-2HAThis study
        ΔeseD/pJN-eseD strainΔeseD mutant with pJN105-eseDThis study
        ΔeseE strainPPD130/91 with in-frame deletion of eseE15
        ΔeseC ΔeseE strainPPD130/91 with in-frame deletion of eseE and eseCThis study
    E. coli
        DH5αα-ComplementationStratagene
Plasmids
    pRE112Suicide plasmid, pir dependent, Cmr, oriT oriV sacB28
    pJN105Arabinose-inducible gene expression vector, araC-PBAD, Gmr29
    pJN-eseCpJN105 with wild-type eseC, GmrThis study
    pJN-eseDpJN105 with wild-type eseD, GmrThis study
    pACYC184Tetr CmrAmersham
    pACYC-eseC-2HApACYC184 with eseC-2HA, TetrThis study
    pFPV25Vector with promoterless gfp gene32
    pFPV−200–−1bp −200 to −1 of escC gene inserted upstream of a promoterless gfp in pFPV25This study
  • a Kms, kanamycin sensitive; Colr colistin resistant; Amps, ampicillin sensitive; LD50, 50% lethal dose; Gmr, gentamicin resistant; Tetr, tetracycline resistant; Cmr, chloramphenicol resistant; Km: kanamycin.