TABLE 3

Strains and plasmids used in this study

Strain or plasmidCharacteristicsSource or reference
Strains
    Escherichia coli EPI300F mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu)7697 galU galK λ rpsL (Strr) nupG trfA dhfrEpicentre
    B. thetaiotaomicronVPI 5482 with tdk deletion; Gmr FUdRrEric Martens, University of Michigan
    B. thetaiotaomicron ΔglsBDerivative of Δtdk strain with glsB deletion; Gmr FUdRrThis study
    B. thetaiotaomicron ΔglsB::glsBΔglsB strain with complementing glsB + 500 bp of upstream sequence; Gmr ErmrThis study
Plasmids
    pBAD24Arabinose-inducible expression vector; Ampr47
    pBAD24-choAArabinose-inducible expression vector; AmprThis study
    pBAD24-choBArabinose-inducible expression vector; AmprThis study
    pBAD24-choBAArabinose-inducible expression vector; AmprThis study
    pEXCHANGE-ΔtdkCarrying cloned tdk; Ampr Ermr45
    pEXCHANGE-Δtdk ΔglsBCarrying cloned tdk; Ampr ErmrThis study
    pNbu2-bla-ermGbInserts into NBU2 att1 and/or att2 site; Ermr Ampr45
    pNbu2-bla-ermGb-pglsBChromosomal insertion vector; inserts into NBU2 att1 and/or att2 site; Ermr AmprThis study