TABLE 2

Glycine lipids in B. thetaiotaomicron

Predicted size of acyl group (m/z)Mean concn (pmol/109 cells) ± SDc
WTΔglsBΔglsB::glsB
3-OH-15:0/3-OH-methyl 14:0 (314.2)b67.9 ± 23.8aND64.6 ± 16.9a
3-OH-16:0/3-OH-methyl 15:0 (328.2)b252.1 ± 36.3aND137.2 ± 98.1
3-OH-17:0/3-OH-methyl 16:0 (342.2)b429.9 ± 71.6aND223.0 ± 157.0
Total monoacylated glycine749.9 ± 131.70424.8 ± 272
3-OH-methyl 15:0 + 13:0/3-OH-methyl 14:0 + 14:0 (524.4)b223.2 ± 25.5ND169.4 ± 30.9
3-OH-methyl 15:0 + 14:0/3-OH-methyl 14:0 + 15:0 (538.4)b792.1 ± 81.2ND635.4 ± 92.0
3-OH-methyl 15:0 + 15:0/3-OH-methyl 16:0 + 14:0 (552.4)b2,000.3 ± 139.3ND1,372.0 ± 181.3
3-OH-methyl 16:0 + 15:0/3-OH-methyl 15:0 + 16:0 (566.4)b2,602.6 ± 118.9ND1,768.0 ± 259.6
Total diacylated glycine5,618.2 ± 364.903,944.8 ± 563.8
Total acylated pool6,368.104,369.6
  • a Detected in only 2 (out of 3) biological replicates.

  • b Acylated glycine confirmed by MS/MS fragmentation. The acyl group designation is indicative, based on the predicted number of carbons in the lipid species.

  • c ND, not detected.