TABLE 2

Enzymatic properties of ancestral and native ARODs using l-Arg as a substrate at various temperaturesa

Temp (°C)AncARODn0 (activation energy, 41.8 kJ/mol)AncARODn1 (activation energy, 36.2 kJ/mol)AncARODn2 (activation energy, 24.8 kJ/mol)OkAROD (native) (activation energy, 34.3 kJ/mol)
kcat (s−1)Km (μM)kcat/Km (s−1 μM−1)kcat (s−1)Km (μM)kcat/Km (s−1 μM−1)kcat (s−1)Km (μM)kcat/Km (s−1 μM−1)kcat (s−1)Km (μM)kcat/Km (s−1 μM−1)
102.1 ± 0.122.0 ± 2.90.092.87 ± 0.1573 ± 690.0053.4 ± 0.0135 ± 2.10.033.2 ± 0.1172 ± 7.00.02
204.3 ± 0.116.4 ± 1.60.35.7 ± 0.11,008 ± 760.0054.7 ± 0.2119 ± 120.047.2 ± 0.1196 ± 6.20.04
306.9 ± 0.111.3 ± 0.80.69.1 ± 0.1913 ± 510.017.5 ± 0.158.1 ± 2.50.138.6 ± 0.2164 ± 8.40.05
409.0 ± 0.212.3 ± 1.20.712.6 ± 0.71548 ± 2700.00811.1 ± 0.2113 ± 5.30.0919.0 ± 0.8715 ± 580.03
  • a The measurement of enzyme kinetics parameters was performed more than three times. Values are means ± standard deviations.