TABLE 1

Specific activity of ancestral ARODs (AncARODn0, AncARODn1, and AncARODn2) and a native AROD (OkAROD) toward the 20 l-amino acids

SubstrateRelative activity (%; mean ± SD)
AncARODn0AncARODn1AncARODn2OkAROD
l-Arginine100 ± 3.7100 ± 1.7100 ± 4.2100 ± 5.1
l-Lysine72.5 ± 2.47.5 ± 0.416.4 ± 0.215 ± 1.0
l-Histidine4.3 ± 0.4<0.0<0.0<0.0
l-Phenylalanine3.6 ± 0.1<0.0<0.0<0.0
l-Leucine1.6 ± 0.2<0.0<0.0<0.0
l-Methionine0.8 ± 0.2<0.0<0.0<0.0
l-Tryptophan<0.0<0.0<0.0<0.0
l-Isoleucine<0.0<0.0<0.0<0.0
l-Valine<0.0<0.0<0.0<0.0
Glycine<0.0<0.0<0.0<0.0
l-Proline<0.0<0.0<0.0<0.0
l-Alanine<0.0<0.0<0.0<0.0
l-Tyrosine0.6 ± 0.0<0.0<0.0<0.0
l-Cysteine<0.0<0.0<0.0<0.0
l-Glutamine<0.0<0.0<0.0<0.0
l-Asparagine<0.0<0.0<0.0<0.0
l-Serine<0.0<0.0<0.0<0.0
l-Threonine<0.0<0.0<0.0<0.0
l-Aspartic acid<0.0<0.0<0.0<0.0
l-Glutamic acid<0.0<0.0<0.0<0.0