TABLE 6

Bacterial strains and plasmids used in these experiments

Bacterial strain or plasmidRelevant characteristic(s)Source
E. coli strains
    ER2267K12 F′ proA+B+ lacIq Δ(lacZ)M15 zzf::mini-Tn10 (KanR)/Δ(argF-lacZ)U169 glnV44 e14(McrA) rfbD1? recA1 relA1? endA1 spoT1? thi-1 Δ(mcrC-mrr)114::IS10, KmrNew England Biolabs
    JW4080-3Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ, rph-1, Δ(rhaD-rhaB)568, ΔmelA745::kan, hsdR514CGSC, the Coli Genetic Stock Center, Yale University
A. tumefaciens strains
    C58Wild type, virulentLaboratory collection
    C580594C58 with an insertion of pBluescript KS− in Atu0594This study
    C580944C58 with an insertion of pBluescript KS− in Atu0944This study
    C581523C58 with an insertion of pBluescript KS− in Atu1523This study
    C581617C58 with an insertion of pBluescript KS− in Atu1617This study
    C581709C58 with an insertion of pBluescript KS− in Atu1709This study
    C58 bglA::pBluescript KS−C58 with an insertion of pBluescript KS− in bglAThis study
    C58 xynA::pBluescript KS−C58 with an insertion of pBluescript KS− in xynAThis study
    C582575C58 with an insertion of pBluescript KS− in Atu2575This study
    C582596C58 with an insertion of pBluescript KS− in Atu2596This study
    C583025C58 with an insertion of pBluescript KS− in Atu3025This study
    C583093C58 with an insertion of pBluescript KS− in Atu3093This study
    C583 arfA::pBluescript KS−C58 with an insertion of pBluescript KS− in arfAThis study
    C583128C58 with an insertion of pBluescript KS− in Atu3128This study
    C58 pglA::pBluescript KS−C58 with an insertion of pBluescript KS− in pglAThis study
    C583285C58 with an insertion of pBluescript KS− in Atu3285This study
    C583312C58 with an insertion of pBluescript KS− in Atu3312This study
    C583553C58 with an insertion of pBluescript KS− in Atu3553This study
    C583564C58 with an insertion of pBluescript KS− in Atu3564This study
    C583914C58 with an insertion of pBluescript KS− in Atu3419This study
    C584485C58 with an insertion of pBluescript KS− in Atu4485This study
    C58 pglB::pCDF-1bC58 with an insertion of pCDF-1b in pglBThis study
    C58 ΔbglBC58 with an in-frame deletion of bglBThis study
    C58 melA::pBluescript KS−C58 with an insertion of pBluescript KS− in melAThis study
    C585072C58 with an insertion of pBluescript KS− in Atu5072This study
    C585082C58 with an insertion of pBluescript KS− in Atu5082This study
    C585284C58 with an insertion of pBluescript KS− in Atu5284This study
    C588097C58 with an insertion of pBluescript KS− in Atu8079This study
Plasmids
    pBluescript KS−Cloning vector CarbrStratagene
    pBBR1mcs-5Cloning vector GentrKovach et al. (37)
    pCDF-1bCloning vector SpecrNovagen
    pBRbglAbglA cloned into pBBR1mcs-5This study
    pBRxynAxynA cloned into pBBR1mcs-5This study
    pBR3100-3Atu3100-3103 inclusive cloned into pBR1mcs-5This study
    pBRarfAarfA cloned into pBBR1mcs-5This study
    pBRpglApglA cloned into pBBR1mcs-5This study
    pBRpglBpglB cloned into pBBR1mcs-5This study
    pBRbglBbglB cloned into pBBR1mcs-5This study
    pBRmelAmelA cloned into pBBR1mcs-5This study
    pK18Kanr, sacB, pRP4 oriV and oriTSchafer et al. (34)
    pKSbglAbglA cloned into pBluescript KS−This study
    pKSxynAxynA cloned into pBluescript KS−This study
    pKSarfAarfA cloned into pBluescript KS−This study
    pKSpglApglA cloned into pBluescript KS−This study
    pKSpglBpglB cloned into pBluescript KS−This study
    pKSbglBbglB cloned into pBluescript KS−This study
    pKSmelAmelA cloned into pBluescript KS−This study