TABLE 2

Growth of A. tumefaciens parent and mutant strains in liquid medium

Bacterial strainDoubling time (min) growing in:
Lysogeny brothAB glucoseaAB sucroseAB cellobioseAB lactose
C58120 ± 10b170 ± 15195 ± 20190 ± 20190 ± 20
C58 bglA::pBluescript KS−120 ± 10170 ± 15210 ± 30No growthcNo growth
C58 xynA::pBluescript KS−130 ± 10165 ± 15160 ± 30200 ± 20160 ± 30
C58 arfA::pBluescript KS−120 ± 15145 ± 20160 ± 35200 ± 20180 ± 20
C58 pglA::pBluescript KS−120 ± 10160 ± 15220 ± 30120 ± 30170 ± 25
C58 pglB::pCDF-1b120 ± 10145 ± 20210 ± 20190 ± 25190 ± 35
C58bglB110 ± 15145 ± 15190 ± 20No growthNo growth
C58 melA::pBluescript KS−120 ± 10145 ± 15120 ± 30150 ± 30230 ± 30
C58 bglA::pBluescript KS− ΔbglB120 ± 15160 ± 25180 ± 30No growthNo growth
  • a AB minimal medium containing 0.2% of the indicated carbon source and 0.01% soytone as an inducer. A minimum of three replicates was carried out for each growth condition.

  • b Average ± standard deviation of the average.

  • c No growth was observed after 48 h of incubation.