TABLE 1

Comparative assessment of insecticidal proteins in insect feeding assays using susceptible and Cry1F-resistant fall armyworms, Spodoptera frugiperda

SampleDosea (ng/cm2)S. frugiperda colonybTotal no. of insect larvaeNo. of larvae/repeatPhenotypic distribution (%) (mean ± SD)c
Dead1st instar2nd instar3rd instar
Buffer0SS4085.1 ± 11.10 ± 00 ± 094.9 ± 16.8
0Cry1Fa-RR4082.5 ± 5.60 ± 07.5 ± 6.890 ± 5.6
Cry1Da_7690SS40887.5 ± 12.512.5 ± 12.50 ± 00 ± 0
690Cry1Fa-RR408100 ± 00 ± 00 ± 00 ± 0
Cry1Da_76,900SS40897.5 ± 5.62.5 ± 5.60 ± 00 ± 0
6,900Cry1Fa-RR40897.5 ± 5.62.5 ± 5.60 ± 00 ± 0
Cry1B.868690SS40897.5 ± 5.62.5 ± 5.60 ± 00 ± 0
690Cry1Fa-RR40880.0 ± 6.820.0 ± 6.80 ± 00 ± 0
Cry1B.8686,900SS40897.5 ± 5.62.5 ± 5.60 ± 00 ± 0
6,900Cry1Fa-RR40887.5 ± 12.512.5 ± 12.50 ± 00 ± 0
Cry1F.842690SS40890.0 ± 10.010.0 ± 10.00 ± 00 ± 0
690Cry1Fa-RR4080 ± 00 ± 02.5 ± 5.697.5 ± 5.6
Cry1F.8426,900SS40897.5 ± 5.62.5 ± 5.60 ± 00 ± 0
6,900Cry1Fa-RR3980 ± 00 ± 05.1 ± 7.094.9 ± 7.0
  • a Amount of protein sample per surface area of diet in a diet overlay assay.

  • b SS, Cry1Fa-susceptible insect colony; Cry1Fa-RR, Cry1Fa-resistant insect colony.

  • c Average phenotypic distribution of larvae across repeats.