TABLE 2

Non-conservative mutations found in single-colony isolates obtained from serial transfer evolution experiments with S. cerevisiae CEN.PK113-7D on SMD variants lacking individual B vitaminsa

TABLE 2
  • a Mutations that were chosen for subsequent reverse engineering experiments are shown in boldface font. S. cerevisiae strains IMS0731 and IMS0726 evolved for fast myo-inositol- and nicotinic acid-independent growth, respectively, did not reveal non-conservative mutations and were not included in the table.

  • b Uppercase letters indicate the mutations.