TABLE 1

Primers and conditions for PCR of resistance genes

Target genePrimer nameSequenceAnnealing temp (°C)PCR amplification product size (bp)Reference
tet(A)tet(A)-f5′-GCTACATCCTGCTTGCCTTC-3′5721044
tet(A)-r5′-CATAGATCGCCGTGAAGAGG-3′
tet(B)tet(B)-f5′-TTGGTTAGGGGCAAGTTTTG-3′5765944
tet(B)-r5′-GTAATGGGCCAATAACACCG-3′
tet(C)tet(C)-f5′-CTTGAGAGCCTTCAACCCAG-3′5541844
tet(C)-r5′-ATGGTCGTCATCTACCTGCC-3′
aphA1aphA-1a-f5′-AACGTCTTGCTCGAGGCCGCG-3′6567045
aphA-1a-r5′-GGCAAGATCCTGGTATCGGTCTGC-3′
blaCMYblaCMY-f5′-TGGCCGTTGCCGTTATCTAC-3′5587046
blaCMY-r5′-CCCGTTTTATGCACCCATGA-3′
blaIMPblaIMP-f5′-GAATAGAGTGGATTAATTCTC-3′5523247
blaIMP-r5′-GGTTTAAYAAAACAACCACC-3′
blaOXAblaOXA-2-f5′-CAAGCCAAAGGCACGATAGTTG-3′5656147
blaOXA-2-r5′-CTCAACCCATCCTACCCACC-3′
blaPSEblaPSE-f5′-TGCTTCGCAACTATGACTAC-3′5543846
blaPSE-r5′-AGCCTGTGTTTGAGCTAGAT-3′
blaTEM-1blaTEM-1-f5′-CAGCGGTAAGATCCTTGAGA-3′5564346
blaTEM-1-r5′-ACTCCCCGTCGTGTAGATAA-3′
catIcatI-f5′-AGTTGCTCAATGTACCTATAACC-3′5054748
catI-r5′-TTGTAATTCATTAAGCATTCTGCC-3′
floRfloR-f5′-CGCCGTCATTCCTCACCTTC-3′5021548
floR-r5′-GATCACGGGCCACGCTGTGTC-3′